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.com
Volume 07
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14
th
International Conference on
Agriculture & Horticulture
New approach for production of volatile and acephenanthrylene constituents (anti-breast cancer) using
biotic and abiotic elicitors in cell cultures of
Pimpinella anisum
L.
Nermeen M. Arafa
National Research Center, Egypt.
P
impinella anisum
L. (anise) is a worthy aromatic medicinal plant involving medical and pharmacological potentials.
Our study aimed to apply the biotechnological techniques using biotic (yeast) and abiotic (L.phenylalanine) elicitors for
enhancement callus productivity of anise and enrichment their content of biological efficient phytochemical constituents
to be applicable inmedical and pharmacological purposes. Total 15 of volatile constituents and acephenanthrylene skeleton
were detected comparatively in callus cultures of
Pimpinella anisum
L. and were identified by GC-MS. Cytotoxicity of the
extracts for suppression breast cancer cell lines (MCF7) proliferation was evaluated. Leaf, stem and shoot tip
explants
of
in vitro seedlings were examined for callus initiation, shoot tip was the best on medium containing 1mg/l IAA+1mg/l
2.4-D+2mg/l kin. Fresh weight, growth rate and increase value of shoot tip calli were maximum values (18.356±0.269,
2.407±0.038, and 11.237±0.179, respectively) after 4 weeks as the optimal period for sub-culture. Shoot tip calli were
improved by culturing on medium containing either yeast at 0.5, 1.0, 1.5 g/l concentrations or L.phenylalanine at 2, 4,
6 mM/l concentrations. Treatments of yeast (0.5, 1.0, 1.5 g/l) and 2mM/l L.phenylalanine increased callus fresh weight
(17.556±0.195, 21.864±0.428, 26.92±0.440 and 13.734±0.199, respectively) compared to calli grown on medium free
elicitors (control) which recorded 12.996±0.284. Total volatile constituents content were boosted to be 32.38%, 40.96%,
43.51% in treatments of 2, 4, 6 mM/l L.phenylalanine, respectively and 45.45%, 31.09%, 26.01% in treatments of 0.5,
1.0, 1.5 g/l yeast, respectively, in comparison to the control (16.83%). 1,2-Diphenyl-5-(t-butyl) acephenanthrylene was
detected in all treatments at different proportions. Anise callus extracts possessed high positive efficiency against breast
cancer prevalence. Cytotoxic activity of callus extracts was between IC50 of 1.33 μg/ml to IC50 of 2.39 μg/ml. From the
phytocomponents point of view, the percents of acephenanthrylene and volatile constituents in anise callus contributed to
their anticancer influence.
nermeenfouad78@yahoo.comAdv Crop Sci Tech 2019, Volume 07