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Volume 07
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14
th
International Conference on
Agriculture & Horticulture
Enhance growth and biochemical composition of
Nannochloropsis oceanica
, cultured under nutrient
limitation, using commercial agricultural fertilizers
M. Ashour
and
Abd El-Wahab Kamel
National Institute of Oceanography and Fisheries, Egypt
M
icroalgae culture media should be economic, allow for high growth, satisfy the needs of microalgal cells and easy
to prepare. In this study, we evaluate the effect of different media formula prepared from commercial agricultural
fertilizers (CAGF), comparing to F/2 Guillard standard medium as a control medium, on growth (cell density, CD; dry
weight, DWand specific growth rate, μ) andbiochemical composition (lipid, protein, and carbohydrate) of
Nannochloropsis
oceanica
. Comparing to N/P ratio (9.6) and actually quantity (12.36 g/l and 1.29 g/l, respectively) of F/2 standard medium,
six N/P ratios (19.2, 9.6, 9.6, 4.8, 3.2 and 1.6) were prepared from Nitric Acid (N-Nt) or Ammonium Sulphate (N-Am),
as a nitrogen source, with phosphoric acid (P), as a phosphorus source, for culturing media of
N. oceanica
. The results
investigated that some CAGFmedia achieved significant (P ≤ 0.05) growth and biochemical composition higher than F/2.
Comparing to lipid percentage (30.70 %) of F/2, the lipid percentage of
N. oceanica
cultured on different CAGF media
were ranging from 18.40% to 46.12%, depending on nutrient limitation, nitrogen source, N/P ratios and actually atom
concentrations. Finally, the use of CAGF constitutes a viable alternative of F/2 medium to reduce the production costs
N.
oceanica
, the commonly used in marine hatcheries and also other biotechnological applications.
abdelwahab.kamil@yahoo.comAdv Crop Sci Tech 2019, Volume 07