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.com
Volume 07
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14
th
International Conference on
Agriculture & Horticulture
Taxonomic study of bacterial pathogens associated with ornamental plants in Assam
Gaurav Phookan, L.C Bora, P.K Borah and Madhumita C. Talukdar
Assam Agricultural University, India
C
ultivation and export of floriculture products have received considerable interest in recent years due to the sector’s
immense potential to generate employment, income and export services. Assam due to its agro – climatic diversity, is
a potential hub for cultivation of different ornamental plants. But the floriculture industry is being challenged mostly by
different biotic stresses. For effective management of the biotic stress, the causal agents needs be identified, characterized
and studied thoroughly. The taxonomic studies on the bacterial pathogen is least reported fromAssam and other states of
the North-Eastern region of India. The present investigation was made to isolate and determine the taxonomic position
of the pathogenic bacterial isolates associated with flowering and foliage ornamental plants of Assam. 32 samples of 20
ornamental plants were collected from three major districts of Assam, viz., Tinsukia, Jorhat and Kamrup. Bacterial growth
was confirmed in 18 diseased samples, which were then subjected to pathogenicity test in their respective host crops to
satisfy the Koch postulates. Six bacterial isolates, viz., could reproduce the symptoms in their respective hosts, viz.;Gerbera
(
Gerbera jamesonii
), Chrysanthemum (
Dendranthema grandiflora
), Anthurium (
Anthurium andreanum
), Marigold
(
Tagetes erecta
), Dendrobium (Dendrobium sp) and Tuberose (Polyanthus tuberosa) hence were proven pathogenic to
their respective hosts. The morphological, cultural, biochemical and molecular characterization of the bacterial isolates
were done.The phylogenetic tree of the bacterial isolates was constructed to determine the similarity with related strains of
respective genera.The isolateGE (J) isolated fromGerbera (
Gerbera jamesonii
) was confirmed to be Pseudomonas cichorii,
isolate CH(K) isolated from Chrysanthemum (
Dendranthema grandiflora
)was confirmed to be Pseudomonas cichorii as
well, isolate AN(K) isolated from Anthurium (
Anthurium andreanum
) was confirmed to be
Xanthomonas axonopodis
pv.diffenbachiae
, isolate MA(J) isolated from Marigold (
Tagetes erecta
) was confirmed to be
Pseudomonas syringae pv.
tagetis
, isolate DE(J) isolated from Dendrobium (Dendrobiumsp)was confirmed to be Burkholderia gladioli and isolate
TU (J) isolated from Tuberose (Polyanthustuberosa) was confirmed to be
Xanthomonas campestris.
gauravphookan30@gmail.comAdv Crop Sci Tech 2019, Volume 07