11 / 45
Page 77
conferenceseries
.com
Volume 07
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14
th
International Conference on
Agriculture & Horticulture
Transcriptome to identify critical genes involved in flowering and floral organ development
Xiuxin Zhang, Shunli Wang
and
Jingqi Xue
Chinese Academy of Agricultural Science, China
Statement of the Problem
: Tree peony (
Paeonia suffruticosa
Andrews) is a globally famous ornamental flower, with
large and colorful flowers and abundant flower types. However, a relatively short and uniform flowering period
hinders the applications and production of ornamental tree peony. Unfortunately, the molecular mechanism of
regulating flowering time and floral organ development in tree peony has yet to be elucidated.
Methodology &Theoretical Orientation
: Because of the absence of genomic information, 454-based transcriptome
sequence technology for de novo transcriptomics was used to identify the critical flowering genes using re-blooming,
non-re-blooming, and wild species of tree peonies.
Findings
: A total of 29,275 unigenes were obtained from the bud transcriptome, with an N50 of 776 bp. The average
length of unigenes was 677.18 bp, and the longest sequence was 5,815 bp. Functional annotation showed that
22,823, 17,321, 13,312, 20,041, and 9,940 unigenes were annotated by NCBI-NR, Swiss-Prot, COG, GO, and KEGG,
respectively. Within the differentially expressed genes (DEGs) 64 flowering-related genes were identified and some
important flowering genes were also characterized by bioinformatics methods, reverse transcript polymerase chain
reaction (RT-PCR), and rapid-amplification of cDNA ends (RACE). Then, the putative genetic network of flowering
induction pathways and a floral organ development model were put forward, according to the comparisons of DEGs
in any two samples and expression levels of the important flowering genes in differentiated buds, buds from different
developmental stages, and treated buds. In tree peony, five pathways (long day, vernalization, autonomous, age, and
gibberellin) regulated flowering, and the floral organ development followed an ABCE model. Moreover, it was also
found that the genes
PsAP1, PsCOL1, PsCRY1, PsCRY2, PsFT, PsLFY, PsLHY, PsGI, PsSOC1,
and
PsVIN3
probably
regulated re-blooming of tree peony.
Conclusion & Significance
: This study provides a comprehensive report on the flowering-related genes in tree peony
for the first time and investigated the expression levels of the critical flowering related genes in buds of different
cultivars, developmental stages, differentiated primordium, and flower parts. These results could provide valuable
insights into the molecular mechanisms of flowering time regulation and floral organ development.
Xiuxin Zhang et al., Adv Crop Sci Tech 2019, Volume 07