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Volume 4, Issue 3 (Suppl)
Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 94
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries
.com
July 14-15, 2016 Brisbane, Australia
4
th
International Conference on
Plant Genomics
Jagjeet Singh Lore et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)
http://dx.doi.org/10.4172/2329-8863.C1.003Whole genome sequencing of ten pathotypes of
Xanthomonas oryzae
pv.
oryzae
using a combination of
Illumina and PacBio sequencing chemistries
Jagjeet Singh Lore, Neelam Kumari, Rupinder Kaur, Inderjit Singh Yadav, Amanpreet K Arora, Bharat Yadav, G S Mangat
and
Kuldeep Singh
Punjab Agricultural University, India
B
acterial blight (BB) of rice caused by
Xanthomonas oryzae pv. oryzae (Xoo)
is one of the major diseases of rice. The pathogen
is highly variable and the current Xoo population is classified into ten pathotypes in north western India. The ten pathotypes
designated as PbXo-1 to PbXo-10 were differentiated using set of near isogenic lines. None of the genes are effective individually
against these pathotypes, thus necessitating pyramiding of genes for durability of resistance to BB. The ten pathotypes have been
collected from Punjab state of India, which is less than 1.5% of total area the country and started growing rice on large scale from
1970 onward. DNA from single colony from each of the 10
Xoo
pathotypes was isolated and sequenced to more 100x coverage using
Illumina and PacBio platforms. Hybrid assembly of the Illumina and PacBio reads lead to assemblage of seven pathotypes into
single molecule and the remaining three pathotypes assembled into 11-15 contigs. Gene prediction revealed high gene density on
both strands with 5500 genes on an average. More than 50 tRNA genes were identified in the
Xoo
pathotypes which is >95% of the
reference genome. We are analyzing the data for TAL Effector genes which form the majority of the
avr
genes in
Xoo
as well as for
other fitness gene. The comparative analysis of the whole genome sequences of all these ten pathotypes along with the other known
reference genomes can provide insights into
Xoo
strain evolution and may help us in finding novel effector genes controlling the
disease pathway.
Biography
Jagjeet Singh Lore has completed his PhD in Plant Pathology from Punjab Agricultural University, Ludhiana, India. Presently, he is working as Plant Pathologist
(Rice) and actively involved in screening and identification of donors for multiple disease resistance in rice and has identified ten pathotypes of
Xanthomonas oryzae
pv.
oryzae
in Punjab. He has standardized screening protocols for sheath blight and neck blast diseases. He has actively contributed towards the development
of high yielding bacterial blight resistant rice varieties occupying a large area in Northern India. He is working in collaboration with International Rice Research
Institute. He is also a Co-PI in several ad hoc research projects on rice improvement and has published more than 40 research articles in reputed national and
international journals.
jagjeetsingh-pbg@pau.edu