Plant-Genomics-2016_Posters-Accepted-Abstracts

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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 92
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Feiyi Huang, Adv Crop Sci Tech 2016, 4:3 (Suppl)
Molecular cloning and characterization of BcCSP1, a Pak choi (Brassica rapa ssp. chinensis) cold shock
protein gene highly co-expressed underABA and cold stimulation
Feiyi Huang
Nanjing Agricultural University, China
C old shock proteins (CSPs) are a kind of evolutionarily conserved nucleic acid binding protein widely distributed from
prokaryotes to eukaryotes. In this study, BcCSP1, a novel CSP was isolated from a Pak choi stress induced cDNA library by
the rapid amplification of cDNA ends method. This gene had an open reading frame (ORF) of 822 base pairs encoding 273 amino
acids. BcCSP1 contained an N-terminal CSD domain and a glycine rich region interspersed with seven CCHC type zinc fingers
at its C-terminus. Multi alignment and phylogenetic analyses showed that BcCSP1 shared high similarity to AtCSP1 and AtCSP3.
Real-time polymerase chain reaction analysis showed that BcCSP1 was induced and co-expressed under cold stress and abscisic acid
treatments. In addition, a BcCSP1-YFP fusion protein was localized to the nucleus and cytoplasm. These results indicated that BcCSP1
plays an important role in responses to cold and ABA treatments in Pak choi. This work may be useful for future functional analysis
of other CSP genes in Pak choi.
Biography
Feiyi Huang is currently a PhD student at Nanjing Agricultural University in China majoring in Vegetable Breeding and Molecular Biology. Her research focuses on
cold stress and controlling flowering time in non-heading Chinese cabbage. She has published a paper entitled “Molecular cloning and characterization of BcCSP1,
a Pak-choi (Brassica rapa ssp. chinensis) cold shock protein gene highly co-expressed under ABA and cold stimulation” in Acta Physiologiae Plantarum.