Plant-Genomics-2016_Posters-Accepted-Abstracts

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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 90
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Juan Pablo Matte et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)
Acceleration of CRIPSR/Cas9 breeding using flowering stimulation and precision lighting
Juan Pablo Matte1, R Siqueira2, B Jones3 and P Arce-Johnson1
1Pontificia Universidad Catolica de Chile, Chile
2University of Lausanne, Switzerland
3The University of Sydney, Australia
As a renewable resource, the demand for wood products is expected to continue to increase in the future. Compared to annual
crop plants, direct genetic modification of trees species has gained little attention, partially because trees have much longer
lifecycles and tight regulations opposing transgenic use in the field. The newly developed gene editing technologies, such CRISPR/
Cas9 increase the potential for the modification of species. CRISPR/Cas9 has several major advantages over previous transgenic based
approaches and can work alongside conventional breeding programs by directly improving known yield related loci or genes. In this
work, we target reporter genes in Arabidopsis thaliana by using a modified CRISPR/Cas9 system and have added a strong ubiquitous
CaMV35S promoter, driving the Flowering Locus T (FT) gene. Ectopic expression of FT accelerates sexual development. To regulate
the acceleration of flowering time to get viable flowers, we use precision lighting with different ratios of Blue, Red and Far Red light.
The CRISPR/Cas9 mutated plants flower earlier than normal as a result of the ectopic FT expression, resulting in fast recovery of the
second generation (F2) in Arabidopsis. We will use this technology to accelerate breeding in arboreus species.
Biography
Juan Pablo Matte has studied both, Forestry Engineering and Biological Science degree in Pontificia Universidad Catolica de Chile. He has completed his PhD in
2013 and Postdoctoral studies in 2015 from The University of Sydney, Australia. Currently he is an Associate Researcher in the Pontificia Universidad Catolica de
Chile, under the PAI project number 82140040 from CONICYT.