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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 100
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Differential expression and identification of new adult plant resistance genes to leaf rust in Brazilian
wheat cultivar Toropi
Sandra Patussi Brammer1, Alice Casassola2, Marcia Soares Chaves1, Jose Antonio Martinelli3, Paula Regina Kuser-Falcao1, Adhemar Zerlotini Neto1, Francesca
Stefanato4 and Lesley Boyd4
1Brazilian Agricultural Research Corporation, Brazil
2University of Passo Fundo, Brazil
3Federal University of Rio Grande do Sul, Brazil
4National Institute of Agricultural Botany, UK
Wheat leaf rust, caused by Puccinia triticina, endemic of South America, it is an important wheat disease. The Brazilian wheat
cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust and prehaustorial resistance phenotype. The
objective of this study was to identify, characterize and to understand the interaction between P. triticina and Toropi by quantitatively
evaluating the temporal transcription genes related to infection and defense inwheat. RNA-Seq libraries from the Toropi here produced
at 0, 6, 12, 24 hours after inoculation with P. triticina. Three replicates of each inoculated and control libraries were sequenced for
each time point. A comparison to the Chinese Spring (CS42) transcriptome was performed aiming to identify putative genes unique
to Toropi. A total of 58 million bp per library were produced: 10,181 contigs of which 9,156 were common to CS42 and 1,025 were
unique to Toropi expressed only in the inoculated libraries. The expression profiles of 15 selected genes varied over time. Classical
defense genes, including peroxidases, b-1,3-glucanases and an endochitinase were expressed (pre and post haustorial) over 72 hours
infection time course, while induction of transcription of other infection related genes with a potential role in defense, although
variable was maintained throughout. These genes had a role in plant lignification, oxidative stress, the regulation of energy supply,
water and lipid transport and cell cycle regulation. These Toropi specific sequences could represent new resistance candidate genes to
be used in wheat breeding and to guide further functional studies on APR to leaf rust.
Identification of maize grain Fe and Zn homeostasis-associated QTL in two mapping populations
Philip J Kear
Cornell University, USA
Iron (Fe) and zinc (Zn) are essential for plant, animal and human nutrition. Low Fe and Zn in crops and livestock results in their
reduced health, which when consumed over prolonged periods can in turn negatively impact the nutrition of human populations.
Maize is grown worldwide as a staple crop for some and a valuable commodity for others and has the potential to be a useful tool
for targeting the dietary Fe and Zn deficiency among the undernourished poor. Maize’s remarkable global spread is largely due to
the degree of genetic and phenotypic diversity that can be harnessed into adaptation to local conditions. This study was performed
on the Goodman Diversity Panel (GDP) with corresponding analysis of the nested association mapping (NAM) population, to take
advantage of greater statistical power and resolution and to perform joint linkage (JL) and genome-wide association (GWAS) analyses
of quantitative genetic loci (QTL) across 3 temperate and 2 tropical locations. Previous studies that have used a candidate-gene
knockout approach have yielded narrower successes in identifying genetic determinants of ionomic homeostasis, whereas the NAM
JL-GWAS approach, in this study, has borne significant QTL identification. Currently 227 JL ionomic-QTLs have been identified
with successive rounds and accompanying GWAS data we anticipate discovery of good QTL for twenty different elements including
those for Fe and Zn homeostasis. A web interface for browsing the maize co-expression network has been developed for querying
individual genes or large regions of the genome that will shortly become available for public use.
Adv Crop Sci Tech 2016, 4:3 (Suppl)