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Volume 4, Issue 2 J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal
Page 30
Notes:
Livestock Nutrition 2016
July 21-22, 2016
conferenceseries.com
July 21-22, 2016 Brisbane, Australia
2nd International Conference on
Livestock Nutrition
The effect of microbial consortia from wild herbivores on goat rumen fibrolytic activity and browse
fermentation
Fon Fabian Nde and Msimango Nokwethemba Nqobile Philile
University of Zululand, South Africa
Acacia species are often recommended as a protein supplement during the long try season or winter because they are often available
and maintain their protein content throughout the year. However, Acacia species or browses are often limited by their high tannin
concentrations which are also detrimental to fibrolytic microbes hence decrease in digestibility especially in domesticated goats.
Therefore this study evaluated the effect of in vitro inocula from wild herbivores (impala, kudu and giraffe) on in vitro fermentation,
gas production and cellulases activities of domestic goats. Consortia were created by mixing fecal inoculum from impala, giraffe and
kuduwith that of goat (N1 (goat+impala, 1:1), N2 (goat+kudu, 1:1), N3 (goat+giraffe+kudu, 1:1:1) andN4 (goat+giraffe+kudu+impala,
1:1:1:1)). Crude protein enzyme extracts (CPZ) from fresh fecal samples were precipitated by 60% ammonium sulfate and assayed for
exocellulase, endocellulase and hemicellulase activities by incubating with crystalline cellulose, carboxymethyl cellulose and xylan at
38o C with optimum pH of 5.5 to 6.5 for 1, 2 and 48 hours, respectively. In vitro degradability was carried out by transferring 33 ml of
fecal inoculum into 67 ml salivary buffer containing 1 g of Acacia sieberiana and 10% tannin (substrate 6.2% was made up to 10% by
adding 100 μL containing 3.8 mg tannic acid) before incubating for 72 hours at 38oC. Apparent degradability (APD), true degradability
(TD), neutral detergent fibre digestibility (NDFdeg), acid detergent fibre digestibility (ADFdeg), cellulose digestibility (CELLdeg),
hemicellulose digestibility (HEMdeg) and MY were calculated. Manipulation of goat enzyme activities with enzymes from the wild
had a positive (P<0.05) influence on goat fibre degradability. Xylanase and endocellulase activities were highest (P<0.05) in N1 while
N4 showed the highest exocellulase activity. Microbial ecosystem N3 had the highest (P<0.05) TD, NDFdeg and ADFdeg while N1
showed the highest degradability for hemicellulose and cellulose. Microbial yield also varied among the microbial ecosystems but was
highest for N2, goat and N4. It was concluded that microbial activities fromwild herbivores might have introduced newmicrobes that
were able to survive on goat ecosystem and improve its fibrolytic potential in vitro. These results showed that microbial ecosystem
from wild herbivores have a potential to improve browse utilization in domestic goat.
Biography
Fon Fabian Nde has completed his PhD (Ruminant Nutrition) from the University of KwaZulu-Natal (UKZN), South Africa and currently working as a Lecturer at the
University of Zululand (UZ) after serving two years as a Post doctorate Researcher at UNIZULU and UZ. He is a Member of the South African Society of Animal
Science, Golden Key International Honor Society and EAAP. His research is focused on improving forage digestion in ruminants especially with potential fibrolytic
microbes from wild herbivores.
Fon Fabian Nde et al., J Fisheries Livest Prod 2016, 4:2 (Suppl)