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Volume10, Issue 12 (Suppl) J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 77
conferenceseries.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20th International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9th WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Characterization of a microRNA expression signature associated with recurrence in oral cavity
cancer patients
Ashlin Ninibeth Lara Holguin, Joaquín Manzo Merino, Roberto Herrera Goephert and Marcela Lizano Soberón
National Cancerology Institute, México
Oral cavity cancer is one of the most prevalent and lethal cancers worldwide. In this type of cancer the local, regional and
distal recurrence develops in up to 76% of the cases. This is associated with treatment failure, as well as high mortality
rates. Therefore, there is a need to identify biomarkers that allow the recognition of patients at high risk of developing cancer
recurrence. MicroRNA (miRNAs) are short single stranded RNA molecules, which regulate genetic expression at the post-
transcriptional level. Changes in the expression profiles of miRNAs have been associated with diagnosis and prognosis in
different types of cancer. Since miRNAs can be recovered from paraffin embedded biopsies, they could have a potential as
biomarkers to understand the course of the disease. Hence, the aim of this work is to characterize a microRNA expression
signature associated with recurrence, in oral cavity cancer patients. A total of 40 paraffin-embedded biopsies from patients
with oral cavity cancer were analyzed. These biopsies were separated in groups of recurrence (n=16, <1 year) and no recurrence
(n=24, >4 years). Total RNA was extracted and quantified for microRNA microarrays analysis (GeneChip® miRNA 4.0,
Affymetrix). The data are being analyzed to determine the differentially expressed miRNAs between the groups, which will
be validated in public databases, and by qRT-PCR. In addition, the expression levels of some targets at the level of mRNA and
protein will be determined by qRT-PCR and immunohistochemistry.
Biography
Ashlin Ninibeth Lara Holguin has completed her Bachelor’s Degree from the Faculty of Superior Studies Iztacala in Universidad Nacional Autónoma de México,
where she obtained Honorific Mention. She is currently studying her Master’s Degree in Biochemical Sciences from National Cancerology Institute, México in
Universidad Nacional Autónoma de México.
Ashlin Ninibeth Lara Holguin et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110