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Notes:
Volume10, Issue 12 (Suppl) J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 82
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World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20th International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9th WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Investigation of GGT5 and GGT7 mRNA expressions in patients with breast cancer
Sevgi Yardim Akaydin1, Dilek Güngör1, Ece Salihoglu1, Hasan Karanlik2 and Semra Demokan2
1Gazi University School of Pharmacy, Turkey
2Istanbul University, Turkey
An increased risk of breast cancer has been reported in individuals with elevated levels of gamma-glutamyltransferase
(GGT) recently or in literature. Genome research indicated that besides GGT1, the human genome contains additional
related genes or sequences. From the perspective of amino acid sequences, genes with substantial (GGT5 and GGT7) similarity
to GGT1 have been identified. The aim of this study was to evaluate the association of gene expression of GGT5 and GGT7
with the breast cancer. For that purpose, tissue and serum samples were taken from 58 patients diagnosed with breast cancer.
As controls, 8 healthy persons admitted to the clinic for breast reduction surgery were also included to the study. mRNA
expressions of GGT5 and GGT7 in matched-normal and tumor tissues of breast cancer patients and normal tissues of healthy
controls were measured by qRT-PCR method. In addition, GGT activity and glutathione (GSH) levels in serum samples were
measured by spectrophotometric and ELISA methods, respectively. In the patient cohort, mRNA expressions of GGT5 and
GGT7 were increased in tumor tissues than those in matched-normal tissues of the same patients (p<0.0001 and p<0.001,
respectively). 52% and 62% of the patients had shown higher GGT5 and GGT7 gene expression respectively, in tumors
compared to matched-normal tissues. Also, GGT enzyme activity was significantly higher in patients than those in controls
(p<0.05) and it was positively correlated with GSH levels in both controls and patients (p<0.05 and p<0.0001, respectively).
However, we could not find any correlation between neither GGT nor GSH and GGT genes. As far as we know, this is the
first study showing GGT5 and GGT7 mRNA expression levels in the patients with breast cancer. Higher expressions in tumor
tissues indicated the importance of these genes in the breast carcinogenesis. Our studies on protein expressions and then
possible functions of these genes are ongoing in our lab.
Biography
Sevgi Akaydın is currently Professor of Gazi University Faculty of Pharmacy Department of Biochemistry. She earned her MD and PhD degree at Gazi University
Faculty of Pharmacy Department of Biochemistry. She focused on cardiovascular disease, oxidative stress and oxidative biomarkers. Her current research
focuses on DNA repair mechanism in molecular subtypes of breast cancer. She has 35 refereed publications and her h-index is 13. Her published works deal with
cardiovascular disease, oxidative stress, oxidative biomarkers and related proteins in cancer.
Sevgi Yardim Akaydin et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110