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Volume 6
Journal of Infectious Diseases & Therapy
Tropical & Bacterial Diseases 2018
December 05-06, 2018
Page 53
Notes:
conference
series
.com
Annual Conference on
Bacterial, Viral and Infectious Diseases
&
Neglected Tropical Diseases Congress: The Future Challenges
December 05-06, 2018 Dubai, UAE
Seroprevalence of
Brucella agglutinins
in patient with pyrexia of unknown origin attending a tertiary
care rural hospital
Smita Damke
1,2
Jawaharlal Nehru Medical College, India
2
Datta Meghe Institute of Medical Sciences, India
Background & Aim:
Human brucellosis is a zoonosis with worldwide distribution, with great importance in developing countries like
India. The diagnosis of brucellosis is frequently difficult to establish as it mimics many other infectious and non-infectious diseases.
The use of feasible diagnostic tests seems to be great importance for diagnosing human brucellosis. The present study was carried out
to study the seroprevalence of human brucellosis by estimating IgG and IgM by ELISA in central India.
Methodology:
A total of 124 serum samples were collected and processed from April 2016 to March 2017 in tertiary care teaching
hospital on central India. The serum samples of the patients admitted to the hospital with the diagnosis of Pyrexia of Unknown (PUO)
were investigated for detectable IgG and igM antibodies by ELISA. The observance value thus obtained was converted to NovaTec Unit
(NTU) by using the formula according to the manufacturer’s instructions.
Results:
In the present study, from the total of 124 serum samples, ELISA detected presence of IgG antibodies in 12 (9.67%) indicating
chronic infection and IgM antibodies in 28 (22.58%) suggesting acute and recent infection.
Conclusion:
ELISA has the ability to measure two specific immunoglobulins for effective diagnosis and is also a rapid method for
detecting seroprevalence of human brucellosis in the community.
Biography
Smita Damke has completed MBBS in 2007 and MD Microbiology in 2013. She is working in a medical college from last 5 years.
smitasdamke@gmail.comSmita Damke, J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C7-057