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Volume 3, Issue 1 (Suppl)

Toxicol Open Access

ISSN: 2476-2067 TYOA, an open access journal

Toxicology Congress 2017

April 13-15, 2017

April 13-15, 2017 Dubai, UAE

World Congress on

Toxicology and Pharmacology

3, 3-Diindolylmethane inhibits RAS/PI3K/AKT signaling through GPR30 abrogate cell proliferation by

BPA induced female Sprague Dawley rats

S Thilagavathi

and

P Pugalendhi

Annamalai University, India

he present study was aimed to evaluate the antiestrogenic effect of 3, 3 –diindolylmethane (DIM) on bisphenol A (BPA) induced

alteration in estrogen signaling pathway in mammary glands of female Sprague-Dawley rats. BPA (10 µg/kg/bw) administered rat

mammary tissues western blot analysis shows an over expression of GPR30, Ras, Src, PI3K andAkt proteins and immunohistochemical

analysis indicates an over expression of PCNA and no significant changes in ERs. Further, oral administration of DIM (5 mg/ kg/bw)

to BPA treated rats alternative days for the period of 12 weeks reveals that significant decrease in the expression pattern of GPR30, Src,

Ras, PI3K, Akt and PCNA as compared to BPA alone treated rats. The results of our study demonstrate that BPA induces rapid action

via the over expression of proteins in nongenomic estrogen signaling pathway. Oral administration of DIM to BPA treated group

inhibits rapid action of BPA by modulating the proteins of nongenomic estrogen signaling pathway.

Recent Publications :

• Thangarasu Rajakumar, Pachaiappan Pugalendhi, Subbaiyan Thilagavathi (2015) Dose response chemopreventive potential

of allyl isothiocyanate against 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley

rats. Chemico-Biological Interactions 231: 35–43.

Biography

S Thilagavathi is a PhD Research Scholar working on Cancer Biology of in vivo model.

S Thilagavathi et al., Toxicol Open Access 2017, 3:1 (Suppl)