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Volume 8, Issue 1 (Suppl) J Cell Sci Ther
ISSN: 2157-7013 JCEST, an open access journal Stem Cell Research 2017
March 20-22, 2017
March 20-22, 2017 Orlando, USA
8th World Congress and Expo on
Cell & Stem Cell Research
The effectiveness of treatment of patients with luminal formCrohn's disease mesenchymal stromal cells
of the bone marrow – 7 years of observation
Knyazev O1, Kagramanova A1, Fadeeva N1, Boldyreva O1, Lishchinskaya A1, Ruchkina I 1, Babayan A1, Kirova M1, Konoplyannikov A2, Noskova K1, Orlova N1
and Parfenov A1
1Moscow Clinical Research Center, Russia
2Medical Radiological Research Center, Russia
Introduction: Anticytokine therapy with anti-TNF-alpha drugs contribute to the achievement of stable remission of Crohn's Disease
(CD). For the treatment of CD Mesenchymal Stromal Cells (MSCs) are also used.
Objective: To examine the long-term efficacy (7 years) therapy of Mesenchymal Stromal Cells (MSCs) from the bone marrow of
patients with luminal Crohn's Disease (CD).
Materials & Methods: 80 patients with luminal form CD (terminal ileitis, colitis and ileokolit) were divided into two groups. The
first group of patients aged 19 to 58 years old (Me-29) (n=34) received the culture of MSCs under the scheme (0-1-2-3, then every 26
weeks). The second group of patients with CD (n=46) aged 20 to 62 years (ME-28) received standard anti-inflammatory therapy with
5-aminosalicylic acid (5-ASA), glucocorticosteroids (GCS) and immunosuppressive (IS). Evaluation of the effectiveness of therapy
on the level of the index of activity of Crohn's Disease (CDAI<150 point) was carried out at 12, 24, 36, 48, 60, 72 and 84 months after
initiation of therapy.
Results: Among the patients in 1-st group, relapse in the 12 months of observation occurred in 4/36 patients (11.76%). In 2-nd
group, relapse occurred in 5/46 (10.8%) (p=0.82). After 24 months in the 1-st group of patients receiving MSC, relapse occurred in
6/34 (17.6%). In the 2-nd group of patients relapse occurred in 19/27 (41.3%) (p=0.044). After 36 months in 1-st group patients with
a relapse of the disease was in 11/34 (32.3%). In the 2-nd group relapse was 29/46 (63.1%) (p=0.01). After 48 months in 1-st group,
receiving MSCs, relapsed in 15/34 (44.1%). In the 2-nd group relapse was in 33/46 (71.7%) (p=0.023). After 60 months in the 1-st
group relapse was in 19/34 (55.9%). In the 2-nd group relapse was 40/46 (86.9%) (p=0.004). After 72 months in 1-st group relapse
was 25/34 (73.5%). In 2-nd group relapse of the CD occurred in 45/46 (97.8%) (p=0.001). After 84 months in 1-st group relapse CD
occurred in 29/34 (85.3%). In the 2-nd group of patients CD relapse occurred in 46/46 (100.0%) (p=0.011).
Conclusions: MSCs transplantation helps to maintain a long-term clinical remission in patients with luminal crohn's disease
compared with GCS/IS therapy.
Exposure to excess phenobarbital negatively influences the osteogenesis of chick embryos
Yu Yan
Jinan University, China
Phenobarbital is an antiepileptic drug that is widely used to treat epilepsy in a clinical setting. However, a long term of phenobarbital
administration in pregnant women may produce side effects on embryonic skeletogenesis. In this study, we aim to investigate the
mechanism by which phenobarbital treatment induces developmental defects in long bones. We first determined that phenobarbital
treatment decreased chondrogenesis and inhibited the proliferation of chondrocytes in chick embryos. Phenobarbital treatment
also suppressed mineralization in both in vivo and in vitro long bone models. Next, we established that phenobarbital treatment
delayed blood vessel invasion in a cartilage template, and this finding was supported by the down-regulation of vascular endothelial
growth factor in the hypertrophic zone following phenobarbital treatment. Phenobarbital treatment inhibited tube formation and the
migration of human umbilical vein endothelial cells. In addition, it impaired angiogenesis in chick yolk sac membrane model and
chorioallantoic membrane model. In summary, phenobarbital exposure led to shortened lengths of long bones during embryogenesis,
which might result from inhibiting mesenchyme differentiation, chondrocyte proliferation and delaying mineralization by impairing
vascular invasion.
J Cell Sci Ther 2017, 8:1 (Suppl)