stem-cell-research-2017_posters-accepted-abstracts

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Volume 8, Issue 1 (Suppl)

J Cell Sci Ther

ISSN: 2157-7013 JCEST, an open access journal

Stem Cell Research 2017

March 20-22, 2017

March 20-22, 2017 Orlando, USA

World Congress and Expo on

Cell & Stem Cell Research

Generation of high quality iPS cells (super iPS cells) using linker histone H1foo

Keiichi Fukuda, Akira Kunitomi and Shinsuke Yuasa

Keio University School of Medicine, Japan

mbryonic Stem Cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent

stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes,

and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents. Here,

we show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with

Oct4, Sox2, and Klf4 significantly enhanced the efficiency of iPSC generation. H1foo promoted

in vitro

differentiation characteristics

with low heterogeneity in iPSCs. H1foo enhanced the generation of germline-competent chimeric mice from iPSCs in a manner

similar to that for ESCs. These findings indicate that H1foo contributes to the generation of higher-quality iPSCs.

J Cell Sci Ther 2017, 8:1 (Suppl)