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Volume 8, Issue 1 (Suppl) J Cell Sci Ther
ISSN: 2157-7013 JCEST, an open access journal Stem Cell Research 2017
March 20-22, 2017
March 20-22, 2017 Orlando, USA
8th World Congress and Expo on
Cell & Stem Cell Research
A self-assembly peptide nanofiber scaffold containing laminin motif induces cellular survival, proliferation,
migration, attachment, and neuronal differentiation: An in vitro study
Sajad Sahab Negah1, 2 and Ali Gorji1, 2, 3
1Mashhad University of Medical Sciences, Mashhad, Iran
2Shefa Neuroscience Research Center, Iran
3Westfalische Wilhelms-Universitat Munster, Germanyt
Tissue engineering has created significant approaches toward the purposes of regeneration of damaged tissues. A general
approach to construct artificial tissues needs artificial extracellular matrices (ECMs) with functions similar to the native ECMs.
Considerable attempt has been made to combine biologically active molecules into the self-assembling peptide in order to improve
cells growth, survival, and differentiation. In this study, RADA4GGSIKVAV (R-GSIK) was designed as a three-dimensional (3D)
nano-fiber scaffold. The cell adhesion, viability, proliferation, migration, and differentiation of rat embryonic neural stem cells (NSCs)
were investigated. The characterization of the R-GSIK shows an open porous structure and a suitable surface area available for cell
interaction. R-GSIK promoted in terms of the cell adhesion, viability, proliferation, and migration. Additionally, the R-GSIK could
enhance NSCs to differentiate into neuron cells. Moreover, the NSCs injected within R-GSIK had a lower glial differentiation rate
than in the puramatrix. Based on our results, it might be concluded that R-GSIK holds great promise for central nervous tissue repair.
J Cell Sci Ther 2017, 8:1 (Suppl)