skin-diseases-and-microbiology-2016_posters-abstracts

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Volume 4, Issue 7(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 51
Notes:
Skin Diseases & Microbiology 2016
October 03-05, 2016
conferenceseries.com
October 03-05, 2016 Vancouver, Canada
International Conference on
Infectious Diseases, Diagnostic Microbiology &
Dermatologists Summit on Skin Infections
Application of a Indoleamine 2, 3-dioxygenase (IDO) expressing allogenic dermal fibroblast populated
within an acellular skin substitute as a biological wound coverage
Ali Farrokhi
University of British Columbia, Canada
Acute and chronic wounds contribute to increased morbidity and mortality in affected people and impose significant financial
burdens on healthcare systems. Despite of advantages of skin grafts, problems such as complications at the donor site, contracture,
loss of elasticity, sensory impairment and undesirable cosmetic results including hypo- or hyper-pigmentation resulted in emerge
of tissue- engineered alternatives. Among these, acellular dermal matrix (ADM) as an extracellular matrix-based biomaterial
has significant mechanical strength with retained biological activity. Further, repopulating dermal fibroblasts into ADM before
transplantation may help the graft to restore its function by synthesizing essential extracellular matrix components, growth factors
and cytokines, which are important for wound healing. To prepare ready-to-use skin substitute harboring live fibroblasts, it is not
feasible to use autologous dermal fibroblasts and using allogeneic fibroblasts can cause immunologic rejection. Although systemic
immunosuppressive drugs are widely used for prevention of allorejection, their side effects are of main concern. Here, we hypothesized
that application of indoleamine 2, 3-dioxygenase (IDO) expressing allogenic dermal fibroblast populated within an ADM is sufficient
to create an immune-privileged area, within the wound, to protect from rejection while providing a rich source of nutrients and
growth factors by fibroblasts, in addition to ADM which is serving as wound coverage. To test this hypothesis, adms were prepared
using a new detergent–free method, recellularized with IDO-expressing or control fibroblasts, and were transplanted on splinted full
thickness murine skin wounds. Investigating the wound healing process in these mice revealed that ADM significantly enhanced the
wound healing process within three weeks. Application of IDO-expressing fibroblasts reduced infiltration of CD4+IL-17+TH-17 and
CD4+IFN-G+TH-1 immune cells to the grafts. Further, local expression of IDO resulted in decreased allo- response and enhanced
immune-tolerance toward allogenic fibroblasts.
The finding of this study shows a correlation between local expression of IDO by fibroblasts and improved wound healing in an
experimental model of allogeneic skin substitute grafting. Further studies are on the way to investigate whether application of this
pre-made non- rejectable biological skin substitute is a viable option for treatment of chronic wounds.
Biography
In September 2012, I started my PhD in Experimental Medicine Program under supervision of Dr. Aziz Ghahary. I attended the University of Ahwaz, Iran for my BSc. in
Genetic and the University of Tehran, Iran for my MSc. in Cellular and Molecular Biology. My current research interest is the studying application of Acellular Dermal Matrix
in skin wound healing. Because of my previous experience in the research area of stem cell, also I am interested in stem cell biology.
Ali Farrokhi, J Infect Dis Ther 2016, 4:7(Suppl)