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Volume 4, Issue 7(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 49
Notes:
Skin Diseases & Microbiology 2016
October 03-05, 2016
conferenceseries.com
October 03-05, 2016 Vancouver, Canada
International Conference on
Infectious Diseases, Diagnostic Microbiology &
Dermatologists Summit on Skin Infections
In silico design of a hexavalent protein, a potential candidate vaccine against Staphylococcus aureus
biofilm-related infection
Maryam Shahbazi
Shiraz University, Iran
Staphylococcus aureus possessing a pool of virulence factors is responsible for the significant and increasing number of hospital and
community-acquired infections worldwide. Developing a potential vaccine to prevent these life-threatening and drug-resistant
infections would have many advantageous impacts on global healthiness. In this study, considering the biofilm mode of growth and
polymicrobial nature of S. aureus and Candida albicans co-infections, a multivalent protein vaccine was designed. In the first phase,
the prediction of putative antigenic targets of S. aureus and C. albicans was conducted based on data mining and bioinformatic
characterization of their proteins. Various properties of the proteins were evaluated such as subcellular localization, hydrophilicity,
repeat containing modules, beta turns, surface accessibility and number of antigenic determinants. Eventually, 6 proteins AlS, ClfA,
FtmB, SdrE, Spa and Bap were selected. The second phase included various immunoinformatics analyses on their sequences leading
to design of a novel sub-unit hexavalent candidate vaccine. Several potential T cell and B cell epitopes are present in this synthetic
construct and it is expected to strongly induce IFN-gamma production. In conclusion, the amino acid sequence introduced here is
expected to enhance T cell-mediated and humoral responses against S. aureus biofilm-related infections to clear biofilm communities
of S. aureus and intracellular colonies of pathogen as well as planktonic cells and thus reducing colonization and persistence.
Biography
Maryam Shahbazi has completed her PhD program in Bacteriology from Shiraz University in 2016 with the thesis entitled “Design and Synthesis of a Protein Candidate
Vaccine against S. aureus Biofilm Related Infections”. She is a Researcher and has published 6 articles in peer reviewed journals.
Maryam Shahbazi, J Infect Dis Ther 2016, 4:7(Suppl)