skin-diseases-and-microbiology-2016_posters-abstracts

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Volume 4, Issue 7(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 64
Skin Diseases & Microbiology 2016
October 03-05, 2016
conferenceseries.com
October 03-05, 2016 Vancouver, Canada
International Conference on
Infectious Diseases, Diagnostic Microbiology &
Dermatologists Summit on Skin Infections
Skin alterations: Prolonged use of steroids by dermoscopy
Alin Laurentiu Tatu
Dunarea de Jos University, Romania
Objectives: The aims of this study were to investigate if the skin alterations after prolonged use of steroids are highlighted by
dermoscopy.
Methods: Patients with variable facial lesions included as (SIFD) after prolonged use of topical steroids more than nine months
minimum twice weekly were examined clinically and by Dermoscopy.
Results: All patients showed telangiectasias (100%) and dermoscopy revealed linear, tortuous and polygonal vessels.72% of the
patients had dermoscopic features for Demodex folliculorum-follicular plugs and Demodex tails. All the 29% patients with clinical
spinulosus had Demodex dermoscopic features.76% of the patients had clinically visible pustules but by dermoscopy the tiny
infraclinical pustules could be seen better and earlier. 77% of the patients had visible erythema on the face and by dermoscopy all
they had red diffuse areas. The white hairs derived from hypertrichosis were observed at 13% with the naked eye and at 43% by
dermoscopy. The atrophy was clinically visible at 12% patients as a severe skin thinning but dermoscopy revealed also atrophic areas
at another 2 patients as white structure less areas or patches between vessels. The patients with dermoscopic atrophy were using
mometasonefuroat and clobetasol propionate.
Conclusions: The dermoscopic particularity of steroid induced rosacea is the association of white intervascular structure less patches
or areas as a sign of the atrophy and also the early detection of hypertrichosis.
Limitations: The small number of the patients may not accurately reflect the percent of dermoscopic findings.
The in vitro effect of methanolic extract to the leaf of Aloe otallensis exudates on the Leishmania ethiopica
and Leishmania donovani parasite
Nigusse Zerihun Tesfaye
Addis Ababa University, Ethiopia
Background & Objectives: Several plant products have been tested and found to possess antileishmanial activity. The present study
was undertaken to evaluate antileishmanial activity of methanolic extract of Aloe otallensis, which is endemic plant to Ethiopia, on
the promastigot stage of Leishmania aethiopica and Leishmania donovani comparing to standard drugs and also tried to screen its
phytochemical constituent.
Methods: Phytochemical screening was done on methanolic extract of the exudates to the leaf of Aloe otallensis. The serial dilution
of the extract was also evaluated for in vitro antileishmanial activity against Leishmania aethiopica and Leishmania donavani on the
strain of L. aethiopica (LDC/134) and L. donovani (AM 563), which is found from the black lion hospital parasitology unit and the
result was compared to standard drug of Sodium stibogluconate, milfostin and paramomycin.
Result: The extract has an antileishmaniacidal activity with an IC50 of 141 μg per ml on L. ethiopica (LDC/134) and 123 μg per ml
on L. donovani (AM 563). The experimental data shows that relatively it has better activity than paramomycin and milfostin but less
activity than sodium stibogluconate, which is given in Ethiopia as a first line drug. The data analyses was done by pad graph prison
version 5 software after it was read by ELISA redder at the wave length of 650 nm. The phytochemical screening of the exudates of
Aloe otallensis showed the presence of phenol, alkaloid and saponin.
Conclusion: The methanolic extract of exudates of Aloe otallensis has a good antileishmanisis activity relatively to paramomycin and
milfostin and this activity may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the
conformation of which constituent present in much concentration and to know which one have highest role.
J Infect Dis Ther 2016, 4:7(Suppl)