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Regenerative & Precision Medicine 2016
December 1-2, 2016
Volume 7, Issue 3(Suppl) J Tissue Sci Eng
ISSN: 2157-7552 JTSE, an open access journal
conferenceseries .com
December 1-2, 2016 | San Antonio, USA
Global Congress on
Tissue Engineering, Regenerative &
Precision Medicine
Emmanuela Maria Anandarajah et al., J Tissue Sci Eng 2016, 7:3(Suppl)
3D intestinal co-culture analysis of the interaction of secreted proteins from intestinal nematode
parasites with the mucosal habitat
Emmanuela Maria Anandarajah, Dana Ditgen, Jan Hansmann, Eva Liebau and Norbert W Brattig
University of Munster, Germany
Bernhard Nocht Institute for Tropical Medicine, Germany
University Würzburg, Germany
Intestinal nematodes represent multicellular organisms within the gut microbiota, which colonize their habitat for years
and which sustain tolerance mechanisms, thereby containing inflammatory host responses to prevent their expulsion. Of
major relevance, concurrently this parasite’s influence attenuates adverse inflammatory responses associated with autoimmune
diseases like Crohn’s disease and ulcerative colitis. The major effect is attributed to excretory/secretory (E/S) products released
from the parasite affecting and modulating host local immune system. We are investigating E/S proteins from the intestinal
Strongyloides ratti and Trichuris suis for immunomodulatory effects. We here report our preliminary characterization of the
two S. ratti proteins, secreted protein acidic and rich-in-cysteine (Sr-SPARC) and thioredoxin-like protein (Sr-Trx-lp) and the
T. suis E/S protein Ts-Trx-lp. The genes of these proteins were identified, cloned and recombinantly expressed under optimized
conditions. The effect of the secreted parasite proteins on host cells were studied applying a novel in vitro 3D mucosal model
that mimics the in vivo natural intestinal microenvironment. In the 3D co-cultures which comprise human intestinal epithelial
and dendritic cells growing on a collagen scaffold, an initial pro-inflammatory response (TNF-α) after 24 hours was followed
by an increased anti-inflammatory response after 48-72 hours detecting the Th2-type-related cytokines IL-22, IL-10 and TSLP.
Thus, Sr-SPARC, Sr-Trx-lp and Ts-Trx-lp can contribute in the reported immunoregulatory potential of intestinal helminth
infection. 3D intestinal mucosal co-cultures represent a novel appropriate model to investigate the interaction of intestinal
parasites and their released products with the host tissue habitat.
Biography
Emmanuela Maria Anandarajah is currently a PhD student from the Westphalian Wilhelms-University of Munster, Germany and finalizing her thesis at the Bernhard
Nocht Institute for Tropical Medicine in Hamburg, Germany. She has completed her BSc in 2011 and MSc in 2013 at the Westphalian Wilhelms-University of
Munster.