public-health-2017_posters-accepted-abstracts

9 / 24

Page 64
Notes:
conferenceseries.com
March 22-23, 2017 | Rome, Italy
2nd World Congress on
Public Health & Nutrition
Volume 7, Issue 1 (Suppl) J Community Med Health Educ 2017
ISSN: 2161-0711, JCMHE an open access journal Public Health 2017
March 22-23, 2017
COMPARISON OF AUTOPROBIOTIC AND PROBIOTIC IN RESTORING INTESTINAL
MICROBIOTAAND DIGESTIVE FUNCTIONAFTER EXPERIMENTAL DYSBIOSIS IN RATS
Elizaveta V Savochkinaa, Yulia V Dmitrievaa, Anna S Alekseevaa, Luidmila V Gromovaa , Maria P Kotylevab, Elena I Ermolenkob and Alexandr N Suvorovb
aPavlov Institute of Physiology, RAS, Russia
bInstitute of Experimental Medicine, Russia
Statement of the Problem: The interplay between the intestinal microbiota and epithelium is an important determinant of host
health and nutritional status. Although probiotic bacteria are widely used to prevent and treat the intestinal dysbiosis, their effects
on the microbiota and the digestive system compared with indigenous (autoprobiotic) strains have not been investigated. This study
aims to evaluate the condition of microbiota and the activity of intestinal enzymes after correction of experimental dysbiosis in rats
using probiotic (Enterococcus faecium L3) and autoprobiotic (12 strains of E. faecium).
Methodology & Theoretical Orientation: The experimental dysbiosis in rats induced by administration of ampicillin and
metronidazole. Then rats were getting probiotic, autoprobiotic or phosphate buffer (PBS, control-2). In control-1, after administration
of water, rats obtained PBS. At the end of the experiment, samples of epithelium and chyme were analyzed biochemically. Fecal
samples were studied bacteriologically and by real time PCR.
Findings: After using probiotic or autoprobiotic, dyspeptic symptoms and microbiota disorders disappeared faster than in control-2.
Autoprobiotic, but not probiotic, promoted bifidogenic effect and increased populations of E. coli and Fecalobacterium sp. in feces.
Aminopeptidase N activity decreased in the epithelium or tended to decrease in the chyme after use autoprobiotic. Tissue-nonspecific
alkaline phosphatase activity, separated from the total activity by inhibiting levamisole, tended to decrease in the epithelium after use
autoprobiotic, and Intestinal alkaline phosphatase – in the epithelium and the chyme after use autoprobiotic or probiotic. Maltase
activity in the chyme tended to increase after use autoprobiotic, but tended to decrease after use probiotic.
Conclusion & Significance: Specific effects of probiotic and autoprobiotic enterococci on intestinal microbiota and digestion have
been demonstrated. The results may be useful for development of a new therapeutic strategy based on autoprobiotic usage in the
correction of intestinal dysbiosis. The work was supported by the Russian Science Foundation Number: 16-15-10085.
Biography
Savochkina Elizabeth V was graduated in 2016 from the Faculty of Biology in the St. Petersburg State University with the specialization in biology. During
undergraduate and graduate studies she gained research experience in the field of histology at the Department of Histology, Cytology and Immunology. In addition,
she attended a one-year course of "Bioinformatics for Biologists" in the St. Petersburg National Research University of RAS. She is currently working as a junior
researcher of Laboratory of Nutrition Physiology in the Pavlov Institute of Physiology, RAS. She has experience of scientific work with laboratory animals, in
techniques of immunofluorescence and confocal microscopy in identification of the presence of glucose transporters SGLT1 and GLUT2 in the apical membrane
of the rat enterocytes.
Elizaveta V Savochkina et al., J Community Med Health Educ 2017, 7:1 (Suppl)