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Volume 4, Issue 5 (Suppl) J Pharmacovigil 2016
ISSN:2329-6887 JP, an open access journal Pharmacovigilance Congress 2016
September 28-30, 2016
September 28-30, 2016 Toronto, Canada
6th Pharmacovigilance Congress
Mohammed Farhad Ibrahim, J Pharmacovigil 2016, 4:5 (Suppl)
Radical scavenging activity and phytochemical constituent of Tulipa Systola Stapf
Mohammed Farhad Ibrahim
University of Salahaddin, Iraq
Introduction: People living on the mountains of the Kurdistan region in Northern Iraq use large amount of herbs for local traditional
medicine. Among them, T. systola, which grows under and between rocks, is very popular as a herbal anti-inflammatory remedy and
pain-relief; two or three fresh bulbs eaten by the patient particularly during inflammation and birth pain. Tulipa systola Stapf. was
collected in April 2014 on the Korek Mountains in Rewanduz-Erbil/Kurdistan region. The materials were identified and classified
from Education Salahaddin University Herbarium (ESUH) by Dr. Abdullah Sh. Sardar, at the University of Salahaddin, Erbil-Iraq. A
voucher specimen was deposited with the accession number (7201).
Experimental: Tulipa systola roots, leaves and flowers (100 g each) were separately defatted with petroleum ether (500 mL), in an
ultra-sonic bath for 30 min, then macerated for 3h under continuous stirring at room temperature. The procedure was repeated three
times for each part. Defatted roots, leaves and flowers were subsequently separately extracted with ethanol (500 mL) in an ultra-sonic
bath for 30 min, and then macerated for 3h under continuous stirring at room temperature. The procedure was repeated three times
for each part. The mixtures were then filtered and the solvent was removed under “vacuum” in a rotary evaporator to afford crude
ethanol extracts: TR from roots, TL from leaves and TF from flowers, respectively. The concentrated extract TR, TL and TL (1 g),
each extract separately was chromatographed over (MPLC (Isolera) RC18, Methanol/Water; (20:80 – 100% methanol) gradient to
afford different fractions. From further purification of these fractions, some pure bioactive compounds isolated and then identified
by spectroscopic; IR, UV, NMR and MS analysis as, TRD2: (+) 1-O-feruloyl-3-O-p-coumaroyl-glycerol and TRB2: (+) 6-tuliposide A
from Roots, TLW5 and F3: (-) Kaempferol-3-O-rutinoside from both Leaves and Flowers part.
Results: This is the first report about phytochemical constituent of Tulipa sytola Stapf. growing in Kurdistan region Iraq. The
radical scavenging and antioxidant activity of the isolated compounds were evaluated on four tests: DPPH free radical scavenging
activity, ferrous ion-chelating power test, total antioxidant activity, hydrogen peroxide scavenging activity, which were carried out as
described in the literatures. Compared to the reference ascorbic acid the IC50 values of the most active compounds were: (DPPH;
IC50, Ascorbic acid 55 µg/ml > F3 65.4 µg/ml > TRD2 77.1 µg/ml > TRB2 135.5 µg/ml), (hydrogen peroxide scavenging; IC50, F3
36.91 µg/ml > Ascorbic acid 38.37 µg/ml > TRD2 40.83 µg/ml), (TAOC; IC50, Ascorbic acid 57.53 µg/ml > TRD2 81.99 µg/ml > F3
121.08 µg/ml). The significant antioxidant and antiradical activities determined for the different compounds give scientific support to
the traditional use of the plant by the Kurdish people as a popular anti-inflammatory remedy and pain-relief and a great subtend to
become a starting point for in vivo investigation in the next steps.
Conclusions: To the best of our knowledge, the optical active isomer of compound TRD2 (+) 1-O-feruloyl-3-O-pcoumaroyl-glycerol
for the first time had been isolated from Tulipa systola roots. The study of the variety of secondary metabolites occurring in T. systola
as a potential source for natural bioactive chemicals, as well as their precise antioxidant mechanisms, is therefore worthy of being
carried out, and it will be reported in due time.
Biography
Mohammed Farhad Ibrahim is a third year PhD student in Natural Product Chemistry and Drug Discovery from medicinal plants. He was awarded MSc degree at
University of Pavia, Italy. He worked as Supervisor in the department of Cosmetic and Pharmaceutical Products at Xenofarma company.