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Pharma & Clinical Pharmacy Congress 2016
November 07-09, 2016
Volume 5 Issue 4(Suppl)
Clin Pharmacol Biopharm
ISSN: 2167-065X CPB, an open access journal
conferenceseries
.com
November 07-09, 2016 Las Vegas, Nevada, USA
4
th
International
Pharma & Clinical Pharmacy Congress
Adesanwo K Julius et al., Clin Pharmacol Biopharm 2016, 5:4(Suppl)
http://dx.doi.org/10.4172/2167-065X.C1.023Antimicrobial and antioxidant activities of extracts and isolated compound from
Cola nitida
(Vent)
Schott et Endl
seed
Adesanwo K Julius, Ogundele S Bayonle and Akinpelu D A
Obafemi Awolowo University, Nigeria
T
his study was designed to investigate the seed nut of
Cola nitida
for its phyto-constituents, antioxidant and antimicrobial
properties. Dried ground seed nut (2 Kg) was extracted by soaking (trice) in methanol at room temperature for 24 hrs.
Combined filtrate was concentrated with rotary evaporator at 45
o
C. The methanol extract was partitioned with n-hexane,
ethylacetate (ETOAc) and n-butanol. Accelerated Gradient Chromatography (AGC) fractionation of ethylacetate extract and
subsequent purification of fractions afforded the identification of phytochemicals. Structural elucidation of isolated compound
I was done with
1
H and
13
C NMR spectroscopic techniques while compound II was identified with GCMS. The crude extract,
fractions and isolated compound were evaluated for their antimicrobial and antioxidant activities by agar well diffusion and
DPPH radical scavenging methods respectively. Compound I was identified to be caffeine (by its
1
H and
13
CNMR spectroscopic
data and literature) and compound II as n-Hexadecanoic acid (by GCMS analysis). Compound 1 demonstrated antimicrobial
activity against
B. cereus, E. coli
and
P. vulgaris
but low antioxidant activity. However, the methanol and EtOAc etracts showed
good antimicrobial and antioxidant activities. The antioxidant activity of the extracts is attributed to the presence of phenolics.
These findings established the antimicrobial and antioxidant activities of the extract from the seed nut of
Cola nitida
and also
establish the presence of caffeine and n-hexadecanoic acid in the extract.
Biography
Adesanwo K Julius completed his PhD from University of Ibadan, Ibadan, Nigeria and Post-doctoral studies from KwaZuluNatal University (KZNU) Duban West-
Ville South Africa. He is a senior lecturer at Chemistry Department, Obafemi Awolowo University, Ile-Ife, Nigeria. He has to his credit, over 16 publications in reputed
journals.
adesanwojk@yahoo.com julius08@oauife.edu.ng