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Pharma & Clinical Pharmacy Congress 2016
November 07-09, 2016
Volume 5 Issue 4(Suppl) Clin Pharmacol Biopharm
ISSN: 2167-065X CPB, an open access journal
conferenceseries .com
November 07-09, 2016 Las Vegas, Nevada, USA
4th International
Pharma & Clinical Pharmacy Congress
Clin Pharmacol Biopharm 2016, 5:4(Suppl)
Platinum-zoledronate complex blocks gastric cancer cell proliferation by inducing cell cycle arrest
and apoptosis
Minhao Xie, Hui Yang, Ling Qiu and Jianguo Lin
Jiangsu Institute of Nuclear Medicine, China
New platinum complexes with different carrier ligands are developing to overcome the intrinsic or acquired resistance of
the tumors during the therapy. A series of novel dinuclear platinum complexes based on the bisphosphonate ligands have
been synthesized and characterized. For the purpose of discovering the pharmacology and action mechanism of this kind of
compounds, the most potent compound [Pt(en)]2ZL was selected for systematic investigation of the underlying mechanisms
accounting for their anticancer activity. In this study, the human gastric cancer cell lines SGC7901 were selected to investigate
the anticancer effects of [Pt(en)]2ZL on this type of cancer cells. The MTT assay and colony formation assay were used to test the
effect of [Pt(en)]2ZL on the cell viability and proliferation, respectively. The senescence-associated β-galactosidase staining and
immunofluorescence staining were also performed to assess the cell senescence and microtubule polymerization. Fluorescence
staining and flow cytometry (FCM) were used to monitor the cell cycle distribution and apoptosis, and the expressions of related
proteins were further detected withWestern blot. [Pt(en)]2ZL exerted profound cytotoxic and antiproliferative effects on SGC7901
cells, and it also induced cell senescence and abnormal microtubule assembly, indicating the progress of mitotic catastrophe.
Cell cycle arrest and apoptosis induced by [Pt(en)]2ZL were observed with FCM and fluorescence staining. The expressions of
cell cycle regulators (p53, p21, cyclin D1, cyclin E and CDK2) and apoptosis-related proteins (Bcl-2, Bax, caspase 3, PARP and
survivin) were mediated by [Pt(en)]2ZL, resulting in the cell cycle arrest and apoptosis. Therefore, [Pt(en)]2ZL exerted antitumor
effect via the cell cycle arrest in the G1/S phase and the induction of apoptosis.
Community pharmacy and the extended community pharmacist practice roles: The UAE
experiences
Mohamed Baraka
University of Dammam, KSA
The pharmaceutical care and ‘extended’ roles are still not practiced optimally by community pharmacists. Several studies
have discussed the practice of community pharmacy in the UAE and have shown that most community pharmacists only
counsel patients. However, UAE, have taken initiatives to allow and prepare community pharmacists to practice ‘extended’
roles. The aim was to review the current roles of community pharmacists in Abu Dhabi Emirate, United Arab Emirates (UAE).
The objective was to encourage community pharmacists towards extending their practice roles. In 2010, Health Authority
Abu Dhabi (HAAD) surveyed community pharmacists, using an online questionnaire, on their preferences towards extending
their counseling roles and their opinion of the greatest challenge facing the extension of their counseling roles. Following this
survey, several programs have been developed to prepare community pharmacists to undertake these extended counseling
roles. In addition to that, HAAD redefined the scope of pharmacist roles to include some extended/enhanced roles. Abu
Dhabi Health Services (SEHA) mission is to ensure reliable excellence in healthcare. It has put clear plans to achieve this;
these include increasing focus on public health matters, developing and monitoring evidence-based clinical policies, training
health professionals to comply with international standards to deliver world-class quality care, amongst others. Prior to making
further plans to extend community pharmacists’ roles, and to ensure the success of these plans, it is imperative to establish the
views of community pharmacists in Abu Dhabi on practicing extended roles and to gain understanding and information on
what pharmacists see as preferred change strategies or facilitators to change. In an attempt to adapt to the changes occurring
and to the growing needs of patients and to maximize the utilization of community pharmacists’ unique structured strategies
are needed to be introduced to the community pharmacy profession.