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Pharma & Clinical Pharmacy Congress 2016
November 07-09, 2016
Volume 5 Issue 4(Suppl) Clin Pharmacol Biopharm
ISSN: 2167-065X CPB, an open access journal
conferenceseries .com
November 07-09, 2016 Las Vegas, Nevada, USA
4th International
Pharma & Clinical Pharmacy Congress
Clin Pharmacol Biopharm 2016, 5:4(Suppl)
Effects of hypobranchial glands and squid ink protein extracts from three Mediterranean molluscs
on human glioblastoma U87 and HeLa cell line epithelia cervix carcinoma
Chabchoub Ellouze Soufia, Ben Mabrouk Hazem, Sayari Nejia and Marrakchi Naziha
Higher Institute of Medical Technologies of Tunis, Tunisia
Background & Aim: The aim of this study is to evaluate the effects of tree Mediterranean molluscs co-product protein
extracts on human glioblastoma U87 and HeLa cell line epithelia cervix carcinoma. Hypobranchial gland proteins (HGPE) are
extracted from the gastropods Hexaplex trunculus (HT) and Bolinus brandaris (BB). The squid ink proteins are extracted from
the cephalopod Sepia officinalis.
Methods: Proteins are extracted by acetone precipitation. Cell viability is measured using MTT assay. Cell adhesion and
migration are established using fibrinogen as matrix.
Results: Both HGPE HT and BB are non-cytotoxic substances until 20 mg/ML. They decrease by more than 50% at 25 mg/mL.
All HGPE significantly impair migration of U87 cells towards fibrinogen in a concentration dependent manner. Concentrations
for 50% inhibition (IC50) of male and female HGPE HT are of 3.7 and 4 mg/mL, respectively. They are of 4.2 and 5.8 mg/ml
for male and female HGPE BB, respectively. Squid ink proteins block the migration of U87 to fibrinogen in a dose dependent
manner. The IC50 is about 9.2 μg/mL. This supernatant also inhibits cell adhesion U87 on various types of matrices. Inhibitions
are 60% fibrinogen and 25% fibronectin. Similarly, HGPE of both HT and BB inhibits HeLa cell adhesion to fibrinogen at 50
mg/mL. Male and female inhibitions significantly impair at 10 mg/mL and continue until 20 mg/mL. Squid ink proteins inhibit
also HeLa cell adhesion. Inhibition significantly impairs at 10 mg/ML and continues until 30mg/mL.
Conclusion: HGPE HT, HGPE BB and squid ink proteins may have the potential to serve as a model for future anticancer-
drugs development.
Design and synthesis of variety of molecules with specific bioactivity
Mingliang Liu
Chinese Academy of Medical Sciences, China
Our research group has been engaged in the design and synthesis of variety of molecules with specific bioactivity for 30
years. Numbers of series of novel compounds were designed, synthesized and evaluated for their antibacterial, anti-TB or
antitumor activity. In the antibacterial drug development area, we are focused on new quinolones targeting on topoisomerase II,
and two candidate IMB-031124 (Chinfloxacin) and IMB-070593 have been completed for Phase I clinical trials and preclinical
trials in China, respectively. In the anti-TB area, many compounds with totally new structural scaffolds were discovered in
our lab to have nanomolar activity again drug-sensitive and -resistant MTB strains. And in the antitumor area, we are mainly
working on the NO/H2S-releasing non-steroidal anti-inflammatory drugs (NSAIDs) and receptor tyrosine kinase (RTK)
inhibitors. Currently, hundreds of compounds synthesized in our lab are evaluated for their antitumor activity.