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conferenceseries.com
Volume 7, Issue 1 (Suppl) J Clin Exp Pathol
ISSN: 2161-0681 JCEP, an open access journal
Pediatric Pathology & Laboratory Medicine 2017
March 15-16, 2017
March 15-16, 2017 London, UK
12th International Conference on
Pediatric Pathology & Laboratory Medicine
Iron deficiency and thalassemia trait in vitaminB12 deficient patients with normal or lowmean corpuscular
volume
Asma Asif and Nadeem Nusrat
Dow University of Health Sciences, Pakistan
Statement of the Problem: Vitamin B12 is one of the most important vitamins of the B-complex group. Its deficiency is not
always accompanied by macrocytic picture refracted in the form of raised MCV. This rise in MCV can be blunted by concomitant
iron deficiency or thalassemia trait. Due to these coexisting conditions, B12 deficiency can be missed on routine CBC leading to
development of severe complications and few of them can be irreversible like neuropathy. The purpose of this research is to study the
importance of normal or low MCV in vitamin B12 deficiency due to co-existence of iron deficiency or beta thalassemia trait masking
a rise in mean corpuscular volume.
Methodology & Theoretical Orientation: Clinical records of 105 vitamin B12 deficient cases (vitamin B12 less than 200 ng/l) who
had presented with normal or low mean corpuscular volume (MCV less than 95 fl) on complete blood count were determined
from Dow diagnostic research and reference laboratory. Serum ferritin, red blood cell folate level and Hb electrophoresis for beta
thalassemia trait were analyzed in these patients.
Findings: A total of 105 vitamin B12 deficient patients who fulfilled the inclusion criteria were enrolled in this study from which
39 (37.14%) were male and 66 (62.85%) were females. Among them, 36.19% were microcytic with the mean age of 37±16.2 years
and 63.8% were normocytic with the mean age of 41.58±15.65 years. In microcytic group, the percent value of iron deficient, beta
thalassemia trait, combined deficiency of B12, iron and beta thalassemia trait and RBC folate deficient were 52.6%, 34.21%, 7.8% and
2.63%, respectively. In normocytic group, the percent value of iron deficient, beta thalassemia trait and RBC folate deficient were
13.4%, 00% and 11.9%, respectively.
Conclusion: It was concluded that frequency of iron deficiency and beta thalassemia trait are significant in vitamin B12 deficient
cases with normal or lowmean corpuscular volume. It is more common in females having age group of 20-40 years. Index of suspicion
should be kept high and serum ferritin and Hb electrophoresis should be done in all such patients who present with B12 deficiency
anemia with normal or low MCV.
Biography
Asma Asif has completed her MPhil in Hematology from Dow University of Health Sciences in 2016. Currently, she is working at the Jinnah Sindh Medical University
Karachi Pakistan. She has her publications in many reputed journals.
Asma Asif et al., J Clin Exp Pathol 2017, 7:1 (Suppl)