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conferenceseries
.com
Volume 7, Issue 1 (Suppl)
J Clin Exp Pathol
ISSN: 2161-0681 JCEP, an open access journal
Pediatric Pathology & Laboratory Medicine 2017
March 15-16, 2017
March 15-16, 2017 London, UK
12
th
International Conference on
Pediatric Pathology & Laboratory Medicine
Analyses of five nuclear and mitochondrial genes for the identification the old and new world
Leishmania
parasites
Parviz Parvizi
and
Reza Fotouhi-Ardakani
Pasteur Institute of Iran, Iran
Background:
The polymorphism and genetic diversity of
Leishmania
genus have status under discussion depending on many items
from clinical samples, reservoir hosts and vectors.
Objectives:
The objectives are to compare genetic changes and the role of each two enzymatic and mitochondrial genes (COII and
Cyt b
) or housekeeping and nuclear genes (
nagt,
ITS-rDNA and
HSP70
) for accurate identification of
Leishmania
parasites.
Methods:
After DNA extractions, PCR products were sequenced and evaluation of genetic proximity and phylogenetic analysis were
performed using MEGA6 and DnaSP5 software.
Results:
Of the 72 sequences, 54 newhaplotypes fromfive
Leishmania
species were submitted toGenBank.The number of polymorphic
sites was significantly lower as compared to the monomorphic sites.
Conclusions:
The highest and lowest levels of haplotype diversity were observed in
L. tropica
(81.35%) and
L. major
(28.38%).
Tajima’s D index analyses showed that Cyt
b
gene in
L. tropica
species was significantly negative, while COII and
nagt
genes were
produced through evolutionary processes for both
L. tropica
and
L. major
.
Biography
Parviz Parvizi has completed his PhD from the London School of Hygiene and Tropical Medicine (London University) and Natural History Museum (London) in
2004. He was a Full Professor since 2015 and has been appointed as the Head of Parasitology department and also as the Director of Parasitology, Immunology
and Mycology Research Group at Pasteur Institute of Iran. He has published more than 60 papers in reputed journals.
parp@pasteur.ac.irParviz Parvizi et al., J Clin Exp Pathol 2017, 7:1 (Suppl)
http://dx.doi.org/10.4172/2161-0681.C1.031