16 / 37
Page 94
conferenceseries
.com
Volume 8
Journal of Nutrition & Food Sciences
ISSN: 2155-9600
Nutri-Food Chemistry
&
Euro Obesity 2018
September 13-15, 2018
JOINT EVENT
September 13-15, 2018 | London, UK
14
th
Euro
Obesity and Endocrinology Congress
&
17
th
World Congress on
Nutrition and Food Chemistry
Effects of high levels of deoxynivalenol and zearalenone contaminated feeds on urine metabolites of
piglets
Kondreddy Eswar Reddy, Sang Yun Ji, Ki Hyun Kim, Hyun Jung Jung, Jin Young Jeong, Min Ji Kim, Sung Dae Lee
and
Hyun Jeong Lee
National Institute of Animal Science - RDA, Republic of South Korea
D
eoxynivalenol (DON) and zearalenone (ZEN) are common food contaminants produced by Fusarium sp. Mycotoxins are
a potential health hazard because of their toxicological effects on both humans and livestock. The aim of this study is to
investigate, in pigs, the effect of higher concentration of DON and ZEN exposure on urine metabolites. In the present study, 15
eight-week-old piglets were randomly assigned to the following three different dietary treatments for 4 weeks: a control group
(fed a standard diet), and the DON and ZEN groups, fed a diet containing 8 mg/kg DON and 0.8 mg/kg ZEN respectively.
After 4 weeks of treatment, all the pigs were euthanized and urine samples were collected directly from the urinary bladder
using syringe. The metabolites of urine were analyzed using UPLC-Q-TOF MS. Total of 5 metabolites were significantly
different (P<0.05) in DON and ZEN dietary treatments when compared with the control group. The metabolites of N,N-
Dimethylarginine, Prolyl-4-hydroxyproline, 6-methyladenosine, Dihyroxy-1H-indole glucuronide, and Tert-butyl (2Z)-(5-
cyano-4,4,5-trimethyl-2-pyrrolidinylidene) ethanoate were significantly increased in DON exposed group than control group.
Contrarily, three metabolites such as Tert-butyl (2Z)-(5-cyano-4,4,5-trimethyl-2-pyrrolidinylidene), prolyl-4-hydroxyproline,
and N,N-Dimethylarginine, were significantly decreased in ZEN dietary group, but Dihyroxy-1H-indole glucuronide Ⅰ and
6-methyladenosine were increased in ZEN dietary group. We speculate that because of the acute toxicity of DON and ZEN
(particularly DON), we found metabolites in the urine of the treatment groups. Additional studies are needed to investigate the
potential relationships between DON- and ZEN toxicity and higher levels of metabolites excretion from the urine.
Recent Publications
1. ReddyKE et al. (2018) Effects of high levels of deoxynivalenol and zearalenone on growthperformance, andhematological
and immunological parameters in pigs. Toxins. 10(3).pii:E114. Doi:10.3390/toxins10030114.
2. Reddy K E et al. (2018) Deoxynivalenol- and zearalenone-contaminated feeds alter gene expression profiles in the livers
of piglets. Asian-Australasian Journal of Animal Science. 31(4):595-606. Doi:10.5713/ajas.17.0466.
3. Reddy K E et al. (2017) Effects of graded concentrations of supplemental lead on lead concentrations of supplemental
lead on lead concentrations in tissues of pigs and prediction equations for estimating dietary lead intake. PeerJ. 5:e3936.
Doi:10.7717/peerj.3936.
4. Reddy K E et al. (2017) Early weaning with different dietary regimen for calves stimulates later rumen developments,
growth and carcass traits in Hanwoo cattle. Asian- Australasian Journal of Animal Science. 30(10):1425-1434.
Doi:10.5713/ajas.17.0315.
5. Reddy K E et al. (2017) Effect of different early weaning regimens for calves on adipogenic gene expression in Hanwoo
loin at the fattening stage. Livestock Science. 195:87-98. Doi:10.1016/j.livsci.2016.11.014..
Biography
Kondreddy Eswar Reddy obtained his Doctoral Degree from Sri Krishnadevaraya University, Anantapur, India. He currently holds a Research Associate position
at National Institute of Animal Science of Rural Development Administration, Jeonju, Republic of South Korea. His current work focuses on (i) developing ways to
improve quantity and quality of native Hanwoo cattle by using high nutritional diet and (ii) developing toxicity testing methods and screening of animal biomarkers
for potential hazards in feeds. He has total seven and half years of Postdoctoral and Research Professor experience at different reputed institutions in Republic of
South Korea. He has been trained and gained expertise in animal and plant genomics by visiting various laboratories in India and Republic of South Korea. He has
published his research findings in esteemed international journals with high impact factor. He has constantly received several national and international awards and
prestigious fellowships from India and Republic of South Korea.
eswar4uk@gmail.comKondreddy Eswar Reddy et al., J Nutr Food Sci 2018, Volume 8
DOI: 10.4172/2155-9600-C7-072