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conferenceseries
.com
Volume 8
Journal of Nutrition & Food Sciences
ISSN: 2155-9600
Nutri-Food Chemistry
&
Euro Obesity 2018
September 13-15, 2018
JOINT EVENT
September 13-15, 2018 | London, UK
14
th
Euro
Obesity and Endocrinology Congress
&
17
th
World Congress on
Nutrition and Food Chemistry
Notes:
A high fat diet induced obesity modulates melanoma tumour microenvironment in Low density
lipoprotein receptor deficient
LDLR
-/- mouse model
Rafah Oday Al-Zubaidi
1
, Cordula Stover
1
and
Lee Machado
2
1
University of Leicester, UK
2
University of Northampton, UK
I
nflammation and altered immune response are themain features of obesity and contribute greatly to the promotion of obesity-
related metabolic complications, especially cancer development and progression. Adipose tissue expansion is associated with
increased tumour infiltration by regulatory T cells (T-regs) which are critical regulators of the adaptive immune response.
Adipocytes and infiltrating immune cells secrete pro-inflammatory adipokines and cytokines providing a microenvironment
favourable for tumour growth. LDLR-/- mice fed on high fat diet and control diet were subcutaneously injected with 5×105
syngeneic melanoma cells (B16F10). After two weeks, tumours and spleens were dissected. Tumours and bodies were weighed
at endpoint and then the percentage of CD4+CD25+FOXP3+T-regs population among splenocytes was determined by flow
cytometry (FACS). High Fat Diet (HFD) feeding increases solid tumour growth combined with increases in adipose tissues of
LDLR
-/- tumour bearing mice. The percentage of T-regs among spleen lymphocytes was significantly higher in tumour bearing
mice fed on high fat diet compared with those fed on control diet. Obesity may promote tumour progression by favouring an
immune suppressive tumour microenvironment.
Recent Publications
1. Jung J I, Cho H J, Jung Y J, Kwon S H, Her S, et al. (2015) High-fat diet-induced obesity increases lymphangiogenesis and
lymph node metastasis in the B16F10 melanoma allograft model: roles of adipocytes and M2-macrophages. Int J Cancer
136(2):258–70.
2. Balistreri C R, Caruso C and Candore G (2010)The role of adipose tissue and adipokines in obesity-related inflammatory
diseases. Mediators Inflamm. 2010:802078.
3. Calle E E, Rodriguez C, Walker-Thurmond K and Thun M J (2003) Overweight, obesity, and mortality from cancer in a
prospectively studied cohort of U.S. adults. N Engl J Med. 348(17):1625–38.
Biography
Rafah Al-Zubaidi is a PhD student at the University of Leicester, UK, studying the role of high fat diet in melanoma cancer. She has 10 years’ experience in immune
system and medical investigations. She built this experience from teaching and administrative work in both hospital and education institutions. She is interested
in cancer immunology research and the role of consuming high fat diet in the development of cancer and the disease progression. She has passion for exploring,
developing and improving methods to decrease the incidence of different cancers diseases.
ra.oday@yahoo.comRafah Oday Al-Zubaidi et al., J Nutr Food Sci 2018, Volume 8
DOI: 10.4172/2155-9600-C7-072