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conferenceseries
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Volume 4
Clinical Neuropsychology: Open Access
Neuropsychiatry 2018
August 27-28, 2018
August 27-28, 2018 Tokyo, Japan
8
th
Global Experts Meeting on
Advances in Neurology and Neuropsychiatry
The neuroprotective effect of glutamate receptors group II agonists in an animal model of birth asphyxia is
connected with inhibition of caspase independent apoptosis
Ewelina Bratek, A Ziembowicz and E Salinska
Mossakowski Medical Research Centre-Polish Academy of Sciences, Poland
Statement of the Problem:
Hypoxic-ischemic encephalopathy is one of the leading causes of neonatal mortality and permanent
neurological disability worldwide. It was shown recently that mGluR2/3 activation before or after ischemic insult results in
neuroprotection, but the exact mechanism of this effect is not clear.
Aim:
The aim of present study was to investigate whether glutamate receptors group II agonists (mGluR2/3) activation after
hypoxia-ischemia reduces brain damage and inhibits apoptotic processes.
Methodology:
We used an animal model of Hypoxia-Ischemia (H-I) on 7-day old rat pups. Animals underwent unilateral
common carotid artery ligation combinedwith75minhypoxia at 7.4%oxygen. Control pupswere sham-operated (anaesthetized
and left common carotid artery dissected, but not ligated). Animals were injected intraperitoneally with mGluR2 (LY 379268)
and mGluR3 (NAAG) agonists, 1 hour or 6 hours after H-I (5 mg/kg of body weight). We examined the weight deficit of the
ischemic brain hemisphere and the expression of caspase independent apoptosis factors (AIF, HTR/OMI and endonuclease G).
The expression of trophic factors GDNF, BDNF and TGF-beta was also measured.
Results:
Our results show that application of each agonist decreased brain tissue weight loss in ischemic hemisphere
independently on the time of application (from 40% in H-I to 15-20% in treated). Both agonists of mGluR2/3 applied 1 hour
or 6 hours after H-I decreased expression of AIF, HTR/OMI and endonuclease G proteins compared to untreated H-I. The
mGluR2/3 agonists application decreased expression of TGF-beta and increased BDNF and GDNF in the ischemic hemisphere
compared to H-I.
Conclusion:
This study demonstrated the neuroprotective effect of mGluR 2/3 agonists on neonatal hypoxic-ischemic brain
injury. Presented data suggest that this effect is connected with decreasing apoptosis.
Biography
Ewelina Bratek is PhD student in Dept. of Neurochem at Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland. Ewelina Bratek
has published more than 3 papers in reputed journals.
ebratek@imdik.pan.plEwelina Bratek et al., ClinNeuropsychol 2018, Volume 3
DOI: 10.4172/2472-095X-C1-003