nano-congress-2016_posters-accepted-abstracts

12 / 17

Volume 7, Issue 4(Suppl) J Nanomed Nanotechnol
ISSN: 2157-7439 JNMNT, an open access journal
Page 76
Nano Congress 2016
August 01-02, 2016
conferenceseries.com
August 01-02, 2016 Manchester, UK
9th Nano Congress for Next Generation
Magnetite nanoparticles on paper: A platform for the diagnosis of dengue fever by magnetic-ELISA
Greter Amelia Ortega Rodríguez
Center for Applied Science and Advanced Technology of IPN, Mexico
Enzyme-linked immunosorbent assays (ELISAs) are the most widely used methods to detect antibodies. However, they have some
drawbacks. As a result, in recent years magnetic nanoparticles or beads (e.g. magnetites) have been combined with ELISAs to
improve their analytical performance. On the other hand, despite paper-based ELISA are less sensitive than conventional ELISA,
they emerge as suitable platforms to develop disposable devices for point-of-care diagnostic. A novel “magnetic-ELISA”, based on
core-shell magnetite@polydopamine nanoparticles supported on Whatman paper was developed to detect IgM-dengue antibodies.
An affordable procedure to deposit magnetite nanoparticles on paper surfaces (Whatman type-1 and Whatman type-ss903) and, to
conjugate such nanoparticles with anti human-IgMantibodies using polydopamine as linker, is reported. Structural features, magnetic
behavior, coating homogeneity, as well as, the nanoparticles/linked antibodies ratio were determined. The analitycal performance of
“magnetic-ELISA” supported on paper surface was 100 times more sensitive with a 700 times lower limit of detection than traditional
ELISA or using magnetic beads without depositing on paper to detect IgM-dengue antibodies. Additionally, the new system showed
low background, acceptable reproducibility, low-cost, easy manufacturing and effortless and easy handling which are very important,
considering the large number of biological samples to be processed by a laboratory in case of dengue epidemics.
Graphene-edge probes for scanning tunneling microscopy
Jeff T H Tsai, Li-Der Chang and Kevin K W Chu
National Taiwan Ocean University, Taiwan
Fabrication of freestanding graphene-edge probes for scanning tunneling microscopy was demonstrated. Graphene was prepared
by thermal chemical vapor deposition (CVD) from solid carbon sources. A Cu wire acted as the substrate for graphene growth. As
the freestanding graphene probes were fabricated, the process was monitored using a micromanipulator and an optical microscope.
Our previous study of electron emission patterns from a field emission microscope demonstrated the layered structure of the
graphene edge. A single-layer of graphene emitted electrons from a limited number of atoms. We found that the graphene emitters
required careful conditioning to achieve a stable emission current. In this research, such activated graphene probes were applied for
use in scanning tunneling microscopes for surface morphology detection. The preconditioned, multi-layer graphene probe presented
resolution that was comparable to conventional probes. Our study generated a practical method for applying individual freestanding
graphene for surface probe microscopy.
J Nanomed Nanotechnol 2016, 7:4 (Suppl)