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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Symptomological, histological and biochemical alterations in male albino rats in response to exposure
to the immunosuppressor, Cyclosporine A
Marwa K Emara
Alexandria University, Egypt
C yclosporine A (CsA) is probably the strongest and safest known immunosuppressor known so far. The histological and
biochemical alterations associated with exposure to the drug have been investigated. Thirty, 3-month old, male albino rats
of 100-120 g body weight .) were randomly divided into three equal groups. Rats were intubated to stomach with CsA in
olive oil (1:3, v/v) for two consecutive days and escape one day for 21 days. The first and the second groups received a dose of
75 mg CsA/kg . and 50mg/kg ., respectively, while the third group received only olive oil to serve as a control. Half of
the rats in each group were euthanized after 10 days and the remaining half was euthanized on day 21 of the experiment. Both
euthanized and dead animals were immediately dissected, internal organs (liver, kidney, spleen, brain, testes, bone marrow,
femur, skull and heart) grossly examined and specimens taken for histological study. Biochemical analysis was conducted on
blood samples collected from all animals after being anaesthetized immediately before euthanasia. CsA induced concentration-
dependent adverse effects in terms of clinical symptomology, histological alterations and biochemical changes. Clinical
symptoms included body weight loss, appetite loss, ruffled hair, mild diarrhea and anorexia. Mild to moderate congestion
was observed in parenchymatous organs, serosal membranes and cerebral blood vessels with dead rats exhibiting mild
haemothorax and enlarged diffusely congested spleen. The pattern of lesions in dead animals was similar, albeit more severe,
in dead as compared to euthanized animals. Kidneys, testes and heart showed severe diffuse cellular swelling with marked
periacinar to midzonal hepatocellular coagulative necrosis. In contrast, the brain, bone and bone marrow appeared within
histological limits with only lungs showing mild bronchopneumonia. Increasing levels of alanine aminotransferase (ALT),
aspartate aminotransferase (AST), urea and creatinine were consistently observed in all animals exposed to CsA.
Keywords: Cyclosporine A, histological alterations, immunosuppressor.
Biography
Marwa K. Emara is a graduate of the Faculty of Veterinary Medicine in 2002, and earned her MSc degree in pathology in 2013. In between, Ms Emara obtained
a clinical pathology Diploma in 2005, mini MBA from AUC continuous program in 2010, and a Diploma in epidemiology & public health from the High Institute of
Public Health in 2011. She worked as pathology specialist in El Madina Medical Lab, and then worked in UNICEF from 2014 in different development projects about
cancer epidemiology and developing and advancing the primary health in Egypt.
Marwa K Emara, J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-035