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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Characterization and biological activities of some colored food additive complexes
3Ragab Y Sharaf, 2Alaa E Ali and 1Mamdouh S Masoud,
1Chemistry Department, Faculty of Science, Alexandria University, Egypt
2Chemistry Department, Faculty of Science, Damanhur University, Egypt
3Regional Joint Lab, Al Beheira Governorate, Ministry of Health, Egypt
This study aims at improving the safety profile and hazardous effects of colored food additive ligation due to chemical
interaction with heavy metal ions, and predicting the hazard. The study based in mixing of 0.01 M of each ligand and
the same weights of metal chlorides (Cr+++, Mn++, Fe +++, Co++, Ni++, Cu++, Zn++ and Cd++). The structures elucidations and
thermal stability of the prepared complexes were done by IR, ESR and UV-visible, and thermal analysis. The IR spectra of
ligands and their complexes showed a shift for uOH, uC-H, uC=C, uC-C, uN=N, uC-N, uS-O and dN=N due to contribution
of azo group and OH group in complexation(1-2). ESR for cupper complexes showed that the structure of the complexes are
octahedral structure and an isotropic symmetrical structure. The UV-Visible spectra showed that the structure of the prepared
complexes are Oh with high spin nature, and the selective azo compound acts as weak ligands. The thermal stability and
thermal decomposition of ligands and they complexes were obtained from thermal analysis (TG and DTA )(3). In this study,
4 microorganisms representing different microbial categories, two Gram-positive (Streptococcus pyogenes and Staphylococcus
Epidermidis), two Gram negative (Escherichia Coli and Klebsiella pneumoniae) bacteria were used. Three different broadly
antibiotics (Levofloxacin, Vancomycin and Amikacin) are used in this study as references. The study included 24 compounds,
four ligands Sunset yellow, Red 2G, Tartrazine yellow and Newcoccine red, and 20 complexes of different metal ions. All
ligands and their complexes showed no activity with Gram negative bacteria but they have different activities to Gram positive
bacteria(4-5).
Keywords: Newcoccine red, Red 2G, Sunset yellow, Tartrazine yellow, thermal analysis and transition metals
Biography
Ragab Youssef Sharaf is a chemist at the Ministry of Health Laboratory, Damanhour, Beheira Governorate since 2012. He runs a private medical analysis laboratory.
Formerly he was a medical analysis specialist at the Egyptian Red Crescent Blood Bank, and Medical representative at Nile Pharmaceutical Company. He earned
his Master of Science in Analytical Chemistry, Faculty of Science, Damanhour University in 2017, Diploma in Analytical Biochemistry, Faculty of Science, Menofia
University in 2007, and a Diploma in Analytical chemistry, Faculty of Science, Damanhour University in 2012. He is currently enrolled for his PhD in analytical
chemistry at Faculty of Science, Damanhour University. He participated in numerous training courses including waste water treatment, air pollutants control, milk
and its products analysis and fish and meats pollutants. He is a board member of the Egyptian Syndicate of Scientific Professions since 2012, and attended and
organized numerous national conference of water.
Ragab Y Sharaf et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-035