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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Isolation and characterization of a thermophilic, cellulose-degrading Streptomyces griseorubens SH15
strain from agricultural waste compost in Egypt
Shimaa F Ahmed, Magda M Mehanni and Ahmed M Abdel- Hamid
Department of Botany and Microbiology, Faculty of Science, Minia University, El-Minia, Egypt
C ellulose is the main component of plant primary cell wall and is the most abundant carbon source on earth. Biodegradation
of cellulose which constitutes from 33 to 50 percent of lignocellulosic biomass provides a sustainable source for
biofuel production. Cellulosic ethanol provides a cleaner and greener alternative to hydrocarbon fuels. Actinomycetes play
a significant role in deconstruction of lignocellulosic biomass. In the current study, compost samples were collected from
botanical garden of faculty of agriculture Minia University, Minia, Egypt. Thermophilic, cellulose degrading actinomycetes
from compost samples were isolated on culture medium supplemented with amorphous cellulose (carboxy methyl cellulose)
and crystalline cellulose (Avicel) as the sole carbon source at 450C. A total of 15 actinomycete isolates were selected and tested
for their qualitative ability to degrade both CMC and avicel on congo red agar medium. The isolate with the highest cellulase
activity on CMC and avicel was selected for further investigation. The 16S rRNA gene sequence was determined for molecular
classification of the isolated strain. On the basis of phylogenetic analysis of 16S rDNA gene sequences, the isolate was classified
as Streptomyces griseorubens (100% similarity). Identification was further confirmed by taxonomical criteria listed in the
International Streptomycetes Project that aligned the isolated strain to the same species; hence the isolated strain was designated
as Streptomyces griseorubens SH15. Factors affecting the production of cellulolytic enzymes by Streptomyces griseorubens
SH15 were investigated. The highest enzyme activity was obtained at 450C and pH7. Moreover, it exhibited cellulolytic activity
on various lignocellulosic substrates including wheat straw, rice straw and sugarcane bagasse. The thermophilic nature and the
ability of depolymerize cellulose makes the isolated Streptomyces griseorubens SH15 a potential candidate for application in
biofuel production.
Keywords: Actinomycetes, Avicel, cellulase, CMC, congo red, Streptomyces griseorubems.
Biography
Shimaa F. Ahmed is currently a master student in Microbiology (Bacteriology and Molecular Biology) at Department of Botany and Microbiology, Faculty of Science,
Minia University, Egypt, since (2013), and had her BSc. of Microbiology, 2013 from the same Department. She accumulated 3-years of experience as a researcher
in an international project on "Nanotechnological approach for the development and implementation of microbial fuel cell for energy harvesting from waste water",
funded from European Union in associated with Research Development and Innovation (RDI) program, Faculty of Engineering, Minia University. She participated
in a number of regional and international workshops as a member of Organizing Committee.
Shimaa F Ahmed et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-035