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.com
Volume 8
Medicinal Chemistry
ISSN: 2161-0444
Medicinal Chemistry 2018
June 14-15, 2018
June 14-15, 2018 | Barcelona, Spain
10
th
World Congress on
Medicinal Chemistry and Drug Design
Electromembrane extraction combined with capillary electrophoresis for the determination of
metoclopramide and ondansetron in urine samples
Ehsan Sadeghi
and
Ali Reza Fakhari
Shahid Beheshti University, Iran
E
lectromembraneextraction(EME)isasamplepreparationtechniqueinpharmaceutical,chemical,clinicalandenvironmental
analysis. This technique uses electro-migration across artificial liquid membranes for selective extraction of analytes and
sample enrichment from complex matrices. This method has many advantages such as simplicity, rapid, low-cost, low LOD,
high pre-concentration factor and high recovery. In the present work, simultaneous pre-concentration and determination of
two basic drugs namely metoclopramide (MCP) and ondansetron (OSN) were studied using EME as a suitable extraction
method, followed with capillary electrophoresis (CE) using ultraviolet (UV) detection as separation technique. The drugs
were extracted from 4 ml sample solutions, through a supported liquid membrane (SLM) consisting 2-nitrophenyloctylether
(NPOE) impregnated in the walls of a polypropylene hollow fiber, and into a 20mL acidic aqueous acceptor solution resent
inside the lumen of the hollow fiber with a potential difference applied over the SLM. The variables of interest, such as chemical
composition of the organic liquid membrane, stirring speed, extraction time and voltage, pH of donor and acceptor phases
and salt effect in the EME process were investigated and optimized. Under optimal conditions NPOE as SLM, stirring rate of
1000 rpm, 200V potential differences, 20min as the extraction time, acceptor phase HCl (pH 1.0) and donor phase HCl (pH
1.5). After the microextraction process, the extracts were analyzed by CE with optimum conditions phosphate running buffer
(pH 2.0), applied voltage of 20 kV and 25°C. Under the optimum conditions, limits of detection (LOD) and quantification
(LOQ) for MCP and OSN were 2.31-2.68 and 7.72-8.91 ng mL-1 respectively. Pre-concentration factor and RSD for five
replicates of each drug were calculated to be 200 and 4.06-3.93 respectively. Finally, the applicability of this method was studied
by the extraction and determination of these drugs in urine samples with recovery percentages of 87–92%.
sadeghiehsan88@gmail.comMed chem (Los Angeles) 2018, Volume 8
DOI: 10.4172/2161-0444-C1-040