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Volume 9
Journal of Analytical & Bioanalytical Techniques
Mass Spectra & Analytika 2018
September 19-20, 2018
September 19-20, 2018 Singapore
World Congress on
Mass Spectrometry and Analytical Techniques
Steroid analysis in LCMS/MS era and the paradigm shift in clinical laboratories
Reena Desai
The ANZAC Research Institute, Australia
T
he accuratemeasurements of steroids have been a pivot tomany clinical laboratories for better patient care in endocrinology,
metabolic disorder, reproductive health and sports doping. Most commonly used immunoassays have their limitations in
specificity and sensitivity, this has led to the popularity and need to develop steroid assays by LCMS/MS. Currently, many
laboratories have adopted this technique and much more to follow the trend, but analyzing steroids by LCMS/MS has set of
challenges making it very difficult. Our laboratory has developed various multi-analyte methods for steroid analysis casing its
metabolism pathway in a single run without any derivatization and making use of the Atmospheric Pressure Photo-Ionization
source (APPI) as part of our routine assays. Not only does the availability of MS-based steroid assays resolve the problems
of direct steroid immunoassays, but multi-analyte profiling also ushers in a new era of snapshot profiling of the overlooked
importance of steroid pathway fluxes that will broaden the interpretation of clinical studies beyond the standard restricted
single analyte-by-analyte analysis. We have methods for serum, urine and dried blood spot detecting steroids of interest by
LCMS/MS in human and mice samples which helps better understanding for research and clinical trials. It is time that clinical
research and practice operates under equally high standards that have now become feasible. Ultimately, the need for the highest
standards in clinical research and practice will drive us to this paradigm shift.
reenadesai@anzac.edu.auJ Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C2-031