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Volume 9
Journal of Analytical & Bioanalytical Techniques
Mass Spectra & Analytika 2018
September 19-20, 2018
September 19-20, 2018 Singapore
World Congress on
Mass Spectrometry and Analytical Techniques
On-line pre-concentration for determination of glycosaminoglycans in cosmetic samples
Kanokporn Chindaphan, Thumnoon Nhujak, Thasinas Dissayabutra, Kanet Wongravee and Monpichar Srisa-Art
Chulalongkorn University, Thailand
C
apillary Zone Electrophoresis (CZE) was developed for separation and determination of Chondroitin Sulfate (CS),
Dermatan Sulfate (DS) and Hyaluronic Acid (HA). Large-volume sample stacking using an EOF pump (LVSEP) was
coupled with CZE for sensitivity improvement. A Background Electrolyte (BGE) for the separation consisted of 200 mM
NaH
2
PO
4
, pH 4.0 and 200 mM butylamine. In addition, 0.5% w/v Polyethylene Glycol (PEG) was also added to the BGE to
reduce sample adsorption on the capillary, thus improving precision of the system. The separation was performed using an
applied voltage of -16 kV. For quantitative analysis of CS, DS and HA, the standard addition method was used to construct
calibration curves, which were in the ranges of 100-300, 100-500 and 50-250 mg L
-1
, respectively. Limits of detection and
quantitation of the proposed method for determination of CS, DS and HA were 3.0, 5.0, 1.0 mg L
-1
and 10.0, 15.0 and 3.0 mg
L
-1
, respectively. In addition, intra-day (n=5) and inter-day (n=3) precisions were investigated and found that the percentages
of relative standard deviation of the migration time and corrected peak area were about 0.4% and 5.9% (for intra-day) and 0.5%
and 5.4% (for inter-day), respectively. The developed method was first applied for determination of HA in cosmetic samples
and found that the recovery percentages of HA were in the range of 95.3-104.4%, indicating high accuracy of the method.
Therefore, the developed method was reliable and simple without complicated sample preparation. Moreover, this method
will be further applied for determination of the HA in human cerebrospinal fluid for checking and monitoring the HA levels
in patients with brain injury diseases.
kanokporn.kloy@gmail.comJ Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C2-031