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.com
Volume 6
Journal of Infectious Diseases & Therapy
ISSN: 2332-0877
Infection Prevention 2018
December 06-07, 2018
December 06-07, 2018 | Valencia, Spain
14
th
World Congress on
Infection Prevention and Control
Effect of lactoferrin-derived synthetic peptides on
Salmonella Enterica
ATCC 14028 planktonic cells
and biofilms
Nidia Leon-Sicairos
1,7
, Angulo-Zamudio Uriel.
2
, Canizalez-Román Adrian
1,5
, Bolscher Jan G.M.
3
, Nazmi Kamran
3
, Flores-Villaseñor Héctor
1
, Velazquez-
Román Jorge
1
and
Vidal-Graniel Jorge
6
1
CIASaP, Facultad de Medicina, Universidad Autónoma de Sinaloa, México.
2
Facultad de Ciencias Químico Biológicas Universidad Autónoma de Sinaloa Av. de las Américas y Josefa Ortiz, México.
3
University of Amsterdam and VU University, 1081 LA, Amsterdam, The Netherlands.
4
Departmento de Biología Celular, México
5
Hospital de la Mujer de la Secretaria de Salud, México.
6
School Medicine, Emory University, Atlanta, Georgia, USA.
7
Hospital Pediátrico de Sinaloa. Blvd, México
S
almonella enterica
is a Gram-negative bacterium responsible of salmonellosis, a gastrointestinal infection that causes 550
million cases each year, from which 220 million are children less than 5 years. In fact,
S. enterica
is one of four major
etiological agent of diarrhea; worldwide. To cause infection, this bacterium contains a lot of virulence factors such as capsule,
lipopolysaccharide, enterotoxins, and the capacity to forms biofilms. It has been reported that biofilm structure protects
Salmonella fromimmune systemcells and antibiotics action.Therefore, the development of newantimicrobials and anti-biofilms
agents to combat salmonellosis are needed. In this work, we investigated the bactericide and biofilmicide effect of bLF and
the peptides Lactoferricin17-30, D-Lactoferricin17-30, Lactoferrampin265-284, D-Lactoferrampin265-284 and LFchimera on
Salmonella enterica
. Materials andMethods: Strains of
Salmonella enterica
were incubated with bLF, LFcin17-30, D-LFcin17-30,
LFampin265-284, D- LFampin265-284, LFchimera and gentamicin in different concentrations during 2 h. Then, the viability
of cultures was assessed by determination of colony forming unit/ml. To form biofilms, strains of
Salmonella
were incubated
for 12 (mature) or 8 (immature), and then; biofilms were treated with bLF, LFcin17-30, D-LFcin17-30, LFampin265-284,
D-LFampin265-284, and LFchimera, during 4 and 6 h. The effect of treatment on biofilms was assessed by colony counts (CFU/
ml) and by Live/dead biofilms vitality kit. In addition; biofilms were visualized by florescence microscopy and fluorescence
was measured. Results: bLF and synthetic peptides shown bactericide effect on salmonella, but bLF and LFchimera presented a
greater effect. On the other hand bLF and LFchimera affected immature biofilms, but only bLF disruptedmature and established
biofilms. Based in our results LF and LFchimera are alternative approaches that could prevent
Salmonella
colonization of the
human host and perhaps against salmonellosis.
Recent Publications
1. Leon-Sicairos, N., Angulo-Zamudio, U.A., Vidal, J.E., Lopez-Torres, C.A., Bolscher, J.G., Nazmi, K., Reyes-Cortes, R.,
Reyes-Lopez, M., De La Garza, M., and Canizalez-Roman, A. 2014 Bactericidal effect of bovine lactoferrin and synthetic
peptide lactoferrin chimera in
Streptococcus pneumoniae
and the decrease in luxS gene expression by lactoferrin.
Biometals 27: 969-980.
2. Baker, E.N., and Baker, H.M. 2009 A structural framework for understanding the multifunctional character of lactoferrin
Biochimie 91: 3-10.
3. Bolscher, J., Nazmi, K., Van Marle, J., Van 'T Hof, W., and Veerman, E. 2012 Chimerization of lactoferricin and
lactoferrampin peptides strongly potentiates the killing activity against Candida albicans Biochem Cell Biol 90: 378-388.
4. Leon-Sicairos, N., Angulo-Zamudio, U.A., Vidal, J.E., Lopez-Torres, C.A., Bolscher, J.G., Nazmi, K., Reyes-Cortes, R.,
Reyes-Lopez, M., De La Garza, M., and Canizalez-Roman, A. 2014. Bactericidal effect of bovine lactoferrin and synthetic
peptide lactoferrin chimera in
Streptococcus pneumoniae
and the decrease in luxS gene expression by lactoferrin.
Biometals 27, 969-980.
5. 5. Rodriguez, A., Pangloli, P., Richards, H.A., Mount, J.R., and Draughon, F.A. 2006. Prevalence of
Salmonella
in diverse
environmental farm samples. J Food Prot 69, 2576-2580.
Nidia Leon-Sicairos et al., J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C6-053