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Volume 6
Journal of Infectious Diseases and Therapy
ISSN: 2332-0877
Infection Congress 2018
March 01-02, 2018
March 01-02, 2018 Berlin, Germany
5
th
International Congress on
INFECTIOUS DISEASES
J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C1-039
A constructed porcine teschovirus with deleted highly conserved “RNNQIPQDF” epitope of VP1 has
potential to serve as DIVA vector
Arthur Tung-Hsuan Tsai
1
, Chia-Yi Chang
2
and
Fun-In Wang
1
1
National Taiwan University, Taiwan
2
Animal Health Research Institute, Taiwan
D
ifferentiation of infected from vaccinated animals (DIVA) is an important strategy for disease control. Development
of a specific epitope-erased marker vector and accompanying diagnostic serum may improve the efficiency of DIVA.
Porcine teschovirus (PTV) is well suited for serving as an immunization vector due to its: Fecal-oral or intranasal route of
infection; pantropism in swine host; generally low virulence and minor epidemic impact on swine herds worldwide and;
DIVA purpose as proposed above. Here, a mono-specific antibody was found to recognize conserved “RNNQIPQDF” epitope
spanning amino acids 188–196 of capsid protein VP1 of PTV1/PS 34 strain. The epitope was deleted and replaced by a reverse
genetics procedure. The ability of this mono-specific antiserum to differentiate the epitope-erased marker PTV from parental
virus shows its potential to serve as an immunization vector for swine diseases. This is the first report of a DIVA possibility
based on an anti-structural protein antibody of PTV. In general, recombined picornaviruses have DIVA potential on using as
vector-based vaccines
.
d99629004@ntu.edu.tw