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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18
th
International Conference on
August 29-30, 2018 | Toronto, Canada
&
LC-MS analysis of intact amino acids on a novel mixed-mode HPLC column
Itaru Yazawa
Imtakt Corporation, Japan
T
here are four established methods for analyzing amino acids: pre-labeled, post-labeled, ion-pairing reversed phase and
normal-phase, but each of these methods has disadvantages. The pre-labeled method has problems with derivitization
efficiency and cost, while the post-labeled method is usually not compatible with LC-MS due to non-volatile mobile phases.
The ion-pairing reversed-phase method has difficulty separating polar amino acids; on the other hand, the normal-phase
mode has problems separating all the compounds, especially the Leu and Ile isomers. We have developed a novel amino acid
separation column for LC-MS (MS) which can separate all 20 amino acids in protein using a mixed-mode stationary phase
structure. We have also estimated separation and detection characteristics using LC-MS instruments. We found two methods
to successfully analyze the complete array of 20 amino acids: 1) high throughput separation with Leu/Ile separation in 5min
and 2) simple gradient separation. We also found that detection can occur not only in single MS mode but also in triple MS
mode. In addition, no derivitization is required and a standard LC-MS (MS) system is sufficient for the analysis. This novel
HPLC method will be a powerful tool for amino acid LC-MS(MS) analysis in many different biochemistry applications.
Biography
Itaru Yazawa has co-founded a company, Imtakt Corporation in 1999 at Kyoto Japan to focus on separation technology providing his own designed and manufactured
HPLC columns to the global market. He used to work for Shimadzu Corporation (instrument development) and YMC Co.Ltd (column development) and then he
wanted to supply his own unique column products such as RP+AX+CX muli-mode ODS column "Scherzo C18 Family" and 2um Non-porous ODS column "Presto
FF-C18" etc. which are based on his own technical idea. Now he will introduce a next-generation novel amino acid analysis column for LC-MS "Intrada Amino Acid"
for this conference.
yazawa@imtakt.comItaru Yazawa, J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-028