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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18
th
International Conference on
August 29-30, 2018 | Toronto, Canada
&
What about dried blood spot for cannabinoid quantification?
Lounès Haroune
Pharmacology Institute of Sherbrooke, QC
W
hile there has been a growing interest in understanding the pharmacological and physiological properties of cannabinoids
in the last decades, analytical methodologies including sample preparations, remain one of the most challenging topics
for their quantification in biological matrices. Moreover, the low sample weight or volume coupled to the complexity of
biological samples (i.e. whole blood, plasma, etc.) could overwhelm the analyst expectations.
In this study, we explored different possibilities to quantify a mixture of 8 natural phytocannabinoids present in biological
samples (cannabinol, cannabigerolic acid, cannabinochromene, cannabigerol, cannbidiolic acid, tetrahydrocanninol,
tetratracannabinolic acid and cannabidiol).
The evaluation was carried-out using plasma and whole blood samples using different usual extraction protocols (solid phase
extraction, liquid-liquid extraction, protein precipitation and blood spot sampling). Stability of tested molecules was also
evaluated in several matrices (plasma, serum,
ex vivo
and pharmacokinetic profiles).
The results showed a moderate matrix effect resulting by signal suppression (≤30%) and acceptable recoveries (≥60%) for most
of the different tested extractions and matrices, except for whole blood when using acetonitrile for protein precipitation, which
appears to be the less efficient approach for cannabinoid extraction, with a recovery lower than ≤40%.
The applicability of tested methodologies was also applied for the determination of pharmacokinetic profiles and showed
that dried blood spot sampling (DBS) could become an interesting alternative for
in vivo
studies. DBS is a rapid, acute and
minimally invasive technic based on a single blood drop (10µL–25µL) that reduces handling and quantity of blood to be
sampled, which consequently also reduces the cost of analysis. According to these aspects, DBS could become a reference
methodology for
in vivo
pharmacokinetic experiments.
Biography
Lounès Haroune is manager of the bioanalytical platform of the pharmacology institute of Sherbrooke University. After 5 years as analytical development manager
in an analytical laboratory and graduated in analytical chemistry, he is working on the development of analytical methodology and sample preparations for the
detection and quantification of biomolecules in biological matrices. He also works on the metabolomics and peptidomics methodologies in complex matrices. He
also focuses on the development and implementation of new analytical workflow for molecular characterizations (biocatalysis, physico-chemistry reaction, etc).
lounes.haroune@USherbrooke.caLounès Haroune, J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-028