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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18th International Conference on
August 29-30, 2018 | Toronto, Canada
&
Determination of enrofloxacin and its metabolite in eggs by capillary electrophoresis together with Fabric
Phase Sorptive Extraction(FPSE)
Yintang Zhang1, Haizhu Jiang1,2 , Yuanqiang Hao1, Fang Chen1, Xiuhua Wei 1, Shusheng Zhang2 and Maotian Xu 1,2
1Shangqiu Normal University, PR China
2ZhengZhou University, PR China
Enrofloxacin is a kind of fluoroquinolones, widely used in the veterinary treatment of diseases. Its residues in eggs pose a risk
to consumers. Fabric Phase Sorptive Extraction (FPSE), a novel sorptive microextraction technique, was used to reduce the
interference of the sample matrix and the loss of analytes in the sample pretreatment process. The quantitative analysis of enrofloxacin
and its metabolite ciprofloxacin was carried out by capillary zone electrophoresis. The separation conditions were optimized: the
running buffer solution was a 40 mM phosphate solution at pH 7.6, the detection wavelength at 254 nm, the separation voltage at
10 kV, the injection time 15s and the sample solvent was a buffer solution diluted 100 times. The linear range of enrofloxacin and
ciprofloxacin was 0.5-10 μg/ml and the detection limits were 0.33μg/mL and 0.17 μg/mL, respectively. The recoveries of the samples
were between 94.4-114.3%. This study extends the application of FPSE in capillary electrophore ciprofloxacin analysis.
J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-028