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Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18
th
International Conference on
August 29-30, 2018 | Toronto, Canada
&
Characterization and distinction of species the genera
Gnaphalium
and
Achyrocline
using chromatographic
profiles (HPLC-DAD) and pattern recognition techniques
Sergio Cuervo-Escobar, Lizeth Lopez-Carrillo
and
Fabian Ladino-Torres
University of Applied and Environmental Sciences, Colombia
P
lants of the genera
Gnaphalium
and
Achyrocline,
belonging to the family
Asteraceae
are used in various parts of the world as
medicinal plants due to their recognized applications in the therapeutic treatment of various pathological conditions. Additionally,
it has been found that its extracts have activities such as antioxidants, anti-inflammatory, antimicrobial and even anti-tumor. Several
species belonging to these genera have similar morphological characteristics which lead to sometimes confused between these genera,
there are, however, significant differences in the class and quantity of flavonoids and other molecules with biological potential present
in them. In this work a chromatographic study was performed by HPLC-DAD, for the leaf and flower ketone extracts in the species
Gnaphalium elegans, Achyrocline satureioides
and
Achyrocline bogotensis
, through which it was possible to establish signal patterns,
specific for each one of them, thus allowing to have a tool for its rapid identification and that it can be used to evaluate the biological
potential of other species the family
Asteraceae
. Through pattern recognition techniques, it was established that these profiles show
significant differences between them, which allows a quick and unambiguous characterization of these species. The methodology
developed for the establishment of chromatographic profiles, which includes a gradient with 2% acetic acid and methanol, on a RP-18
column, It also allows the identification and quantification of at least 10 flavonoids that may be present in these species. These results
contribute to the optimization of time and resources around the investigations that will lead to the establishment of therapeutic
treatments from extracts of these plants.
scuervo@udca.edu.coJ Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-028