20 / 25
Page 82
Journal of Analytical & Bioanalytical Techniques | ISSN: 2155-9872 | Volume 9
World HPLC, Separation Techniques & Pharmacovigilance
World Analytical Chemistry & Mass Spectrometry
18
th
International Conference on
August 29-30, 2018 | Toronto, Canada
&
The effect of glycerol on shape, size and growth rate of
Escherichia coli
Maryam S. Hosseini-Zare
Texas Southern University, USA
C
arbohydrate is a decisive factor in the growth of bacteria. Studies showed the importance of glucose as the most common carbon
source of bacteria in the bacterial growth. However, the role of glycerol on bacterial growth remained to be clear. In the current
study, we examined the effects of glycerol on the two strains (HF19, MC1061) of
Escherichia coli
growth. To this aim, we measured
the optical density and colony formation unit per milliliter of cultured media. In addition, the cell dry mass of the cultured media has
been assessed. Furthermore, in order to directly study the effect of glycerol on the
E. coli
cell proliferation, we used hemocytometer.
Here we found strong correlation between optical density and CFU/ml in the all incubation period in the control groups. Surprisingly,
our data showed very weak correlation between optical density and CFU/ml in the glycerol-treated group after 24-hour incubation.
Our data also indicated that glycerol increased the optical density, colony formation unit and cell dry mass of the sample. Although
our data indicated strong relation in the control groups, in all incubating period, in the glycerol-treated group, we observed strong
relation only in 6-hour and 12-hour incubation period, not in 24-hour incubation. This data suggest that in long time glycerol
incubation, glycerol induced changes in shape and/or size of
E. coli
in both strains.
maryamhoseinizare@gmail.comJ Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C1-028