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Volume 7
Journal of Traditional Medicine & Clinical Naturopathy
Herbal Traditional 2018
December10-11, 2018
Page 50
Notes:
December10-11, 2018 Dubai, UAE
International Conference on
Herbal & Traditional Medicine
In vitro
antioxidant and antisickling effects of
sennaalexandrina
on sickle cell
Dunya Ahmed Nori
1,
Alyousef Z
1
, Choudhry, H
1
Filimban, F
1
Elassouli M Z
2,
Helmi N
3
1
King AbdulAziz University Saudi Arabia;
2
King Fahad Mediacl Research Center, Saudi Arabia
3
University of Jeddah, Saudi Arabia
S
ickle cell disease (SCD) is one type of blood diseases related to autosomal disorder 1. The sickle shaped red blood cells are
the main cause of many problems in the blood vessels and capillaries1,2. Many natural plants have been used in treatment
of sickle cell anemia and they listed as antisickling agents in several researches3,4
Sennaalexandrina
(S) is a medicinal plant
that has many popular uses, and has been proved its efficacy 5,6. The aim of this study was to assess the antioxidants activity
and the antisickling effect of (S) extractions.
Method:
Air-dried leaves of (S) plant were grinded and the active components were extracted by maceration in water and
methanol as solvents. The antioxidants activity of (S)-water and (S)-methanol were assessed by way of the radical scavenging
method using 2,2-diphenyl-1-picrylhydrazyl (DPPH).To determine the antisickling effect of (S) extracts; 20 samples were
collected from sickle cell anemia patients. Different concentrations of (S) extracts (500 and 1000 μg/mL) were added on the
sample and incubated. A drop of each sample was examined with light microscope.Normal and sickled RBCs were calculated
and expressed as the percent of sickling. The stabilization effect of the extracts was measured by the osmotic fragility test for
erythrocytes Pauline et al., (2013)7.
Result:
As estimated by DPPH method, (S)-water and (S)-Methanol extracts revealed an antioxidants activity by 87.71
and 97.71 % respectively compared to the 98.03 % of control. Sickle cells treated with extracts at different concentrations
significantly reduced the percentage of sickling compering to control samples. However, (S)-Methanol at 1000 μg/mL give the
highest anti-sickling affect while (S)-Water at 1000 μg/mL showed the highest membrane cell stability.
Conclusion:
The results showed that (S) extracts have antisickling effects. Therefore, the
Sennaalexandrina
may has a role in
SCD management and a good impact on the patient’s lives.
Biography
Dunya Ahmed Nori completed his Master degree in Science at the age of 29 years from king Abdu Alaziz university in 2017 with excellent degree in Biochemistry
field. She got the Bachelor degree from the same university in the same field in 2013 with excellent degree - first honors. She has published two papers in reputed journals.
dany667@hotmail.comDunya Ahmed Nori
et al., J Tradit Med Clin Natur 2018, Volume 7
DOI: 10.4172/2573-4555-C4-012