herbal-traditional-2018-posters-abstracts

2 / 8

conferenceseries.com
Volume 7 Journal of Traditional Medicine & Clinical Naturopathy Herbal Traditional 2018
December10-11, 2018
Page 44
Notes:
December10-11, 2018 Dubai, UAE
International Conference on
Herbal & Traditional Medicine
The potential protective effect of both honey and olive oil against the methotrexate induced
hepatotoxicity in rats: Biochemical, histological and immunohistological study
Hani Abdulfattah AL-Turkistani
King Abdulaziz University, Saudi Arabia
Background: Both honey and olive oil are natural products that are exhibited a good reputation not only because of their high
nutritional values but also for their therapeutic properties. A lot of cytotoxic drugs are widely used in the treatment of different
types of malignancies, which targeting the proliferation ability of the tumor cells. However, their use leads to injury of the
normal cells. Among these drugs is the methotrexate (MTX), which is used in the treatment of a wide range of malignancies
and autoimmune diseases. However, it produces many side effects that may even threaten the patient's life e.g. hepatotoxicity.
The aim of the work: Is to evaluate the potential effectiveness of single and combined administration of honey and olive oil in
the protection from the MTX-induced hepatotoxicity in rats.
Method: Eighty four adult male albino rats were used in the present study. They were divided into 7 equal groups; group I was
a negative control group, group II was given freshly prepared honey orally by gavage daily at a dose of 1.2 g/kg , group III
was given olive oil (2 ml/day), group IV was given a single intraperitoneal injection of MTX (20 mg/kg bwt), group V was given
MTX + freshly prepared honey orally, group VI was givenMTX as above + olive oil, group VII was givenMTX as above + honey
+ olive oil. At the end of the experiment (3 weeks), the rats of different groups were sacrificed and blood samples were collected
for the determination of the liver function parameters (liver enzymes, protein profile and bilirubin). Then, the abdomen of
rats of different groups was opened where pieces of the liver were excised. Half of these pieces were homogized to measure the
oxidative (malondialdehyde [MDA]) and antioxidative parametes (superoxide dismutase [SOD], Catalase [CAT] and glutathione
peroxidase [GP-x]); while the other pieces were processed for different histological and immunohistochemical techniques.
Result: Administration of honey and olive oil exerted a protective effect against MTX-induced hepatotoxicity as demonstrated
by normalization of the liver enzymes, proteins and total bilirubin and histopathological and immunohistological changes
observed in the liver. In addition, both agents also reverse the oxidative damage of the liver by decresing MDA level and
increasing activities of the antioxidant enzymes in the liver homogenates, as compared with control rats. These effects were
more evident when the two agents were given together.
Conclusion: These results provide new evidences of the hepatoprotective effect of combined intake of honey and olive oil on
the biochemical and structrural MTX-induced liver damage indicating synergistic effect between them. Consequently, co-
administration of these agents might be an effective aduvant therapy in cancerous patients given chemotherapy to alleviate its
side effects. Furthermore, we believe that the outcome of this study will represent an important opportunity to get use of these
valuable natural products, which were mentioned in many holy Quranic verses.
Biography
Bachelor of Science degree in microbiology: College of Applied of Sciences, UmmAl- Qura University, Makkah 2003 . Master of Science (MS) in Technical Anatomy
& Histology Faculty of Medicine, King Abdul-Aziz University, Jeddah . Now in the final stage of the study of the Faculty of Science King Abdulaziz University2018.
Publications include: Publication of an article ,The Museum of Anatomy ,World of Words Magazine , University of Toronto , Toronto ,Canada . 2015 :Alturkistani
HA1, Tashkandi FM, Mohammedsaleh ZM (2015) Histological Stains: A Literature Review and Case Study , Pup Med , US National Library of Medicine National
Institutes of Health. He also work as a supervisor in the anatomy department of King Abdulaziz University, Jeddah, Saudi Arabia
Hani Abdulfattah AL-Turkistani, J Tradit Med Clin Natur 2018, Volume 7
DOI: 10.4172/2573-4555-C4-012