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Volume 8, Issue 10 (Suppl) J Diabetes Metab
ISSN: 2155-6156 JDM, an open access journal Herbal Diabetes 2017
November 02-04, 2017
November 02-04, 2017 Bangkok, Thailand
23rd International Conference on
Herbal and Alternative Remedies for
Diabetes and Endocrine Disorders
Protective antioxidant effects of polyphenols extracted from the French medicinal plant Antirhea
borbonica on cerebral endothelial cells exposed to diabetes-related hyperglycemia
Angelique Arcambal, Janice Taile, Anne Bialecki, Olivier Meilhac and Marie-Paule Gonthier
University of Reunion, France
Type-2 diabetes promotes vascular complications, leading to neurological disorders such as stroke. Indeed, hyperglycemia
alters the blood-brain barrier integrity by deregulating the cerebral endothelial cell function. Oxidative stress may
play a causal role. Thus, the biological effect of plant polyphenols known to exert antioxidant capacities is of high interest.
We evaluated the effect of polyphenols from the medicinal plant Antirhea borbonica referred in the French Pharmacopeia
for antidiabetic properties, on the production of redox and vasoactive markers from cerebral endothelial cells exposed to
hyperglycemia. Polyphenols extracted from Antirhea borbonica were identified by UPLC-MS method. Then, their action on
murine bEnd.3 cerebral endothelial cells exposed to hyperglycemia was determined by measuring the intracellular levels of free
radicals (DCFH-DA assay), SOD activities (enzymatic assay) and the production of redox and vasoactive molecules (RT-qPCR,
DAF-FM assay). We found that Antirhea borbonica exhibited a high content (4%, w/w) of polyphenols including caffeic acid,
chlorogenic acid, kaempferol and quercetin. Plant polyphenols decreased hyperglycemia-induced production of free radicals
and NADPH oxidase 4 gene expression. Moreover, plant polyphenols counteracted the deregulation of Cu/ZnSOD activity and
Nrf2 redox transcriptional factor gene expression mediated by hyperglycemia. Preconditioning of cells with specific inhibitors
targeting the signaling molecules JNK, ERK, PI3K and NFκB modulated hyperglycemia-induced oxidative stress and showed
their possible involvement in polyphenol action. Polyphenols also abrogated hyperglycemia-mediated down-regulation of the
intracellular levels of NO vasodilatator. Interestingly, caffeic and chlorogenic acids detected among the major polyphenols of
Antirhea borbonica exerted similar protective effects. Collectively, these findings demonstrated that polyphenols extracted
from Antirhea borbonica protected cerebral endothelial cells against hyperglycemia-mediated oxidative stress. Further studies
are in progress to evaluate the in vivo benefits of plant polyphenols on a mouse model exposed to hyperglycemia and middle
cerebral artery occlusion to mimic a cerebral ischemia during type-2 diabetes.
Biography
Angelique Arcambal is persuing her PhD from University of Reunion, France, under the supervision of Professor Marie-Paule Gonthier. Marie-Paule Gonthier is
a Professor of Nutrition at the Medicine School of the University of Reunion in France. She has received her PhD degree in Nutrition from the Medicine School of
Clermont-Ferrand, France. Her work consisted on evaluating the bioavailability of dietary antioxidant polyphenols in humans and demonstrated the role of the gut
microflora on polyphenol metabolic fate. During her Post-Doctoral position at the National Research Center of Naples, Italy, she contributed to the understanding of
the effects of endocannabinoids derived from dietary lipids on adipose tissue biology and reported the overproduction of endocannabinoids from human adipocytes
and pancreatic beta cells during obesity and type-2 diabetes.
Angelique Arcambal et al., J Diabetes Metab 2017, 8:10 (Suppl)
DOI: 10.4172/2155-6156-C1-072