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.com
Volume 8, Issue 10 (Suppl)
J Diabetes Metab
ISSN: 2155-6156 JDM, an open access journal
Herbal Diabetes 2017
November 02-04, 2017
November 02-04, 2017 Bangkok, Thailand
23
rd
International Conference on
Herbal and Alternative Remedies for
Diabetes and Endocrine Disorders
In vitro
and
in vivo
hypoglycemic evaluation of
Terminalia chebula
Retz leaves
Jayashree Dutta and M C Kalita
Gauhati University, India
D
iabetes mellitus is one of the fast-growing health problems in both developing and developed nations. A wide and diverse
range of plants reported in Indian literature are used in the treatment of diabetes mellitus. Being a store house of medicinal
plants, the north-eastern part of India serves as reservoir of traditional based knowledge for treatment of several diseases
including diabetes. In the present study, the leave part of
Terminalia chebula
Retz was accessed for its hypoglycemic potentiality
both
in vitro
and
in vivo
. Initial in vitro test was performed using enzyme α-amylase and α-glucosidase.
In vitro
analysis was
followed by
in vivo
hypoglycemic evaluation in alloxan induced diabetic rat model.
leaves has demonstrated
a moderate level of α-amylase inhibition (70.46%) with IC50-06.09±0.342 mg/mL and very high yeast α-glucosidase inhibition
(100%) with IC50-0.956±0.342 mg/mL compared to standard reference drug acarbose. Oral carbohydrate tolerance test of
methanol extract
T. chebula
leaves revealed that oral administration of the extract at the dose of (300 mg/kg b.w) to maltose
loaded diabetic rats significantly (P<0.05) suppressed the rise of post-prandial blood glucose level compared to the standard
drug acarbose. The phytochemical analysis reveals that the crude methanol extract of
T. chebula
leaves is very rich in
phytoconstituents like phenol, tannin flavonoids, terpenoid and glycosides compared to petroleum ether extract and acetone
extract. The total phenol and flavonoid content of
T. chebula
leaves was found to be 123.64±1.09 mg/g and 184.23±2.34 mg/g,
respectively. The study concludes that the leaf part of
T. chebula
is a potential inhibitor of enzyme α-glucosidase that can be
employed for further for development of suitable anti-diabetic formulation working against postprandial hyperglycemia.
Biography
Jayshree Dutta is a Research Scholar in Department of Biotechnology, Gauhati University, India. She has been working in the field of anti-diabetic medicinal plants
from last five years. She has completed her Masters in Biotechnology and is very much interested in the emerging field of pharmaceutical biotechnology leading to
drug discovery. She had earlier documented few plants having hypoglycemic potentiality from North East region of India via tremendous field survey.
jshrdtt@gmail.comJayashree Dutta et al., J Diabetes Metab 2017, 8:10 (Suppl)
DOI: 10.4172/2155-6156-C1-072