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.com
Volume 8, Issue 5 (Suppl)
J Blood Disord Transfus, an open access journal
ISSN:2155-9864
Hematology 2017
November 08-09, 2017
November 08-09, 2017 | Las Vegas, USA
11
th
International Conference on
Hematology & Hematological Oncology
Altered avidity to α and β antigenic reactions induced by malaria parasitaemia Nigerian subjects
Chinedu Kingsley Dike
and
Olutayo Ifedayo Ajayi
University of Benin, Nigeria
P
lasmodium falciparum
infected erythrocytes have been reported to display several dramatic morphological changes
that affect membrane integrity such as rigidity, antigenic character and permeability. These modifications occur both at
the erythrocyte cytoskeleton and extracellular surface of the membrane resulting in sub-cellular modifications of adhesive
properties exhibited by the affected cells. It is plausible therefore to hypothesize possible alterations of blood group antigenic
reactivity during the infection process that could cause danger in serological procedures. Our objective therefore was to
ascertain the possible changes in the reactivity of α and β antigens to their corresponding antibodies in parasitized erythrocytes.
A total of 200 blood samples comprising of 50 each from parasitemic subjects of blood groups A and B respectively. They were
compared with 50 samples each from control subjects from corresponding blood groups respectively who tested negative for
plasmodiasis. Confirmatory tests for malaria parasites were done by two algorithms of microscopy and rapid diagnostic tests.
Standard tile and tube methods were used for direct and reverse blood grouping techniques with washed and unwashed red
cells while time taken for agglutination reactions to take place was recorded as a score of avidity of the antibodies used on
the red cell antigens. We recorded a significantly reduced reaction times in malaria parasitized red cells compared with non-
parasitized controls in both blood groups A and B (P<0.05, respectively). Also, there were statistically reduced reaction times
in unwashed cells compared with washed cells in both test and control erythrocytes (P<0.05, respectively). The reaction times
using sera from subjects and controls on standard cells during reverse grouping were equally affected. We hereby conclude
that, irrespective of density of parasitemia, reaction times of α and β antigens with their corresponding antibodies are reduced
significantly. This could lead to errors in serological interpretations with malaria infected red cells especially during emergency
cross match and with less avid sera. The continuous use of washed red cells for serological procedures is equally re-emphasized.
Biography
Chinedu Kingsley Dike holds a Bachelor’s degree in Medical Laboratory Science with a bias in Clinical Chemistry. He is currently pursuing Post-graduate degree
in Medical Laboratory Science. His main research interest is immunological studies of hemoparasites.
bensonlaurence@yahoo.comChinedu Kingsley Dike et al., J Blood Disord Transfus 2017, 8:5 (Suppl)
DOI: 10.4172/2155-9864-C1-028