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.com
Volume 8, Issue 5 (Suppl)
J Blood Disord Transfus, an open access journal
ISSN:2155-9864
Hematology 2017
November 08-09, 2017
November 08-09, 2017 | Las Vegas, USA
11
th
International Conference on
Hematology & Hematological Oncology
In vitro
and
in vivo
evidences of sickling reversal induced by rehydration with high K+- isotonic solution
Olutayo Ifedayo Ajayi, Chinedu Kingsley Dike, Oludolapo Uchegbu
and
Damilola Agbaminoja
University of Benin, Nigeria
Achievers University, Nigeria
E
rythrocyte sickling and adhesion are favored by cellular dehydration, which increases the rate of hemoglobin polymerization and
cell sickling. Potassium chloride co-transport and calcium-activated potassium channel (Gardos channel) mediate erythrocyte
dehydration in sickle cell disease and β-thalassemia. We investigated the
in vitro
and
in vivo
effects of various concentrations of K+
ions in physiological solutions (PSS) as well as in
Cocos nucifera
water (CNw) which is known for its natural high potassium content
and isotonicity. This study was aimed at ascertain the efficacy of high potassium isotonic solutions in rehydrating sickle cell and
possibly reversing the sickling phenomenon at
in vivo
and
in vitro
situations. Erythrocytes from 20 sickle cell anemia (SCA) as well as
46 healthy subjects were studied. One part was treated with sodium metabisulfite (Na2S5O7) solution to induce maximum sickling
as controls while the other was subjected to different high concentrations of K+ in PSS as well as
Cocos nucifera
water (40 mM, 80
mM and CNw – 65 mmol/L) respectively. The procedure was repeated for the normal HB AA subjects. Also, both groups of subjects
were given 10 ml/kg body weight of coconut water to drink as a single dose for the
in vivo
experiment. Blood samples were collected
longitudinally before and after the oral ingestion at 1 hour and at 24 hours for analysis of red cell indices as well as stained blood
films used to ascertain the percentage sickled erythrocytes count before and after the treatment in both cases. Maximum percentage
counts of sickled cells after the addition of Na2S5O7 (45%) were observed which decreased significantly (P<0.05, respectively) to
about 2% with
Cocos nucifera
and 10% with 80 mM K+ PSS. The count in 40 mM K+-PSS was not statistically significant. In both Hb
AA and SS subjects, MCH and MCV remained relatively stable when compared with the pre-ingestion sample (P>0.05, respectively)
while MCHC increased significantly in both groups as early as 1 hour and sustained till the 24th hour. MCHC was equally raised in
the
in vitro
samples (P<0.05, respectively). The morphology of red cells also indicated a lesser count of sickled red cells after the oral
ingestion.
Cocos nucifera
water and other high potassium ion solutions can activate the rehydration of sickled erythrocytes by probably
de-activating the Gardos channel to increase the mean corpuscular hemoglobin concentration (MCHC) and thereby restoring the
normal red cell shape. We suggest a probable pharmacological value of the
Cocos nucifera
water as well as other formulated high
potassium but isotonic fluids in SCA management.
Biography
Olutayo Ifedayo Ajayi holds a Diploma in Hematology and Blood Transfusion Science and a Fellowship Diploma in Clinical Chemistry from the Medical Laboratory Council
of Nigeria. He later proceeded to University of Benin to study Human Physiology where he bagged his MSc and PhD degrees. He has many publications in both local and
international journals. He is currently an Associate Professor and Head of Department of Physiology at University of Benin in Nigeria.
ifedayo1@yahoo.comOlutayo Ifedayo Ajayi et al., J Blood Disord Transfus 2017, 8:5 (Suppl)
DOI: 10.4172/2155-9864-C1-028